Comparative Study of Chlorogenic Acid Extraction from Different Plants

The objective of this study was to evaluate the efficiency of extraction of chlorogenic acid (CGA) from different plant matrices such as heather (Calluna vulgaris) flowers, green tea (Camelia sinensis) leaves and blueberry (Vaccinium myrtillus) fruits. Ethanol-water mixture was compared with 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) and 1-butyl-3-methyl imidazolium tetrafluoroborate ([Bmim]BF4). The highest CGA content was obtained in heather flowers using 60% EtOH aqueous solution, while [Bmim]Cl ionic liquid proved to be the good solvent for green tea and blueberries matrices. Statistically insignificant differences (p <0.05) were observedwith prolonged ultrasonic time and [Bmim]Cl for blueberry samples, but significant decrease using 60% EtOH and [Bmim]BF4. HPLC analysis showed that CGA was degradated to caffeic acid under these conditions.