Complexation of 5-aminovaleric acid zwitterions in aqueous/methanol solution by heterotopic tri-cationic receptors

Heterotopic tri-cationic receptors based on 4,10,16-triaza-18-crown-6 are capable of efficient and selec-tive binding of the zwitterionic form of 5-aminovaleric acid (5-AVA) in aqueous/methanol solution. Thecooperative participation of both cation and anion binding domains of this receptor in 5-AVA complexa-tion was established by1H NMR experiments and DFT calculations. Heterotopic receptors andfluoresceinwere used for the preparation of indicator displacement assay and this assembly was applied to selectiveoptical sensing of 5-aminovaleric acid.