Efficient propagation of suspended HL-60 cells in a disposable bioreactor supporting wave-induced agitation at various Reynolds number

Growth of human nonadherent HL-60 cell cultures performed in disposable bioreactor under various hydrodynamic conditions of 2-D wave-assisted agitation has been compared and discussed. Influence of Reynolds number for liquid (ReL) and thekLacoefficient, as key parameters characterized the bioprocessing of HL-60 cells inReadyToProcessWAVETM25 system, on reached values of the apparent maximal specific growth rate (μmax) and the specific yield of biomass (YX/S) has been identified. The values of ReL(i.e., 510–10,208), as well askLacoefficient (i.e., 2.83–13.55 h−1), have been estimated for the cultures subjected to wave-induced mixing, based on simplified dimensionless correlation for various presents of WAVE 25 system. The highest values of apparentμmax = 0.038 h−1andYX/S = 25.64 × 108cells gglc−1have been noted for cultures independently performed at wave-induced agitation characterized by ReLequaled to 5104 and 510, respectively. The presented results have high applicability potential in scale-up of bioprocesses focused on nonadherent animal cells, or in the case of any application of disposable bioreactors presenting similitude.