Artykuł w czasopiśmie
Brak miniatury
Licencja

CC-BYCC-BY - Uznanie autorstwa

HPLC-PDA-ESI-HRMS-Based Profiling of Secondary Metabolites of Rindera graeca Anatomical and Hairy Roots Treated with Drought and Cold Stress

Autor
Sykłowska-Baranek, Katarzyna
Pietrosiuk, Agnieszka
Misicka-Kęsik, Aleksandra
Wileńska, Beata
Naliwajski, Marcin Robert
Data publikacji
2022
Abstrakt (EN)

To cope with environmental harmful conditions, plant cells developed adaptive strategy that involves production of a wide variety of complex secondary metabolites. The spectrum and quantity of biosynthesized compounds in specific plant species is determined by its genotype, tissue, developmental and physiological stage and environmental factors. This phenomenon was used to exploit the potential of anatomical and hairy root cultures of Rindera graeca to produce bioactive compounds. Cultivated in vitro roots were subjected to abiotic stresses i.e., drought or coldness. Next the extract profiling was performed using HPLC-PDA-ESI-HRMS method, as well quantitative determination of caffeic, rosmarinic and lithospermic B acids, that were present in all root extracts. Phenolic acids, flavonoids and iridoids represent the major groups of compounds detected in chemical profiles growing under various conditions roots. The highest number of phytochemicals was determined in roots subjected to coldness. Lithospermic B acid proved to be the most abundant compound in all investigated extracts. Among applied abiotic stress factors it was demonstrated that coldness affected to the most secondary metabolites production. The results of current study suggest that root cultures of R. graeca could serve as a new and abundant source of lithospermic B acid.

Słowa kluczowe EN
Abiotic stresses
Boraginaceae rosmarinic acid
Chemical profile
In vitro cultures
Lithospermic B acid
Total phenolic and total flavonoid content
Dyscyplina PBN
nauki chemiczne
Czasopismo
Cells
Tom
11
Zeszyt
6
Strony od-do
931
Data udostępnienia w otwartym dostępie
2022-03-08
Licencja otwartego dostępu
Uznanie autorstwa