Cryoprotection and banking of living cells in a 3D multiple emulsion-based carrier

The ability to preserve stem cells/cells with minimal damage for short and long periods of time is essential for advancements in biomedical therapies and biotechnology. New methods of cell bank- ing are continuously needed to provide effective damage prevention to cells. This paper puts for- ward a solution to the problem of the low viability of cells during cryopreservation in a traditional suspension and storage by developing innovative multiple emulsion-based carriers for the encap- sulation and cryopreservation of cells. During freezing-thawing processes, irreversible damage to cells occurs as a result of the formation of ice crystals, cell dehydration, and the toxicity of cryo- protectant. The proposed method was effective due to the “flexible” protective structure of multi- ple emulsions, which was proven by a high cell survival rate, above 90%. Results make new con- tributions in the fields of cell engineering and biotechnology and contribute to the development of methods for banking biological material.