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Alterations in Primary Carbon Metabolism in Cucumber Infected with Pseudomonas syringae pv lachrymans: Local and Systemic Responses

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cris.lastimport.scopus2024-02-12T20:33:16Z
dc.abstract.enThe reconfiguration of the primary metabolism is essential in plant–pathogen interactions. We compared the local metabolic responses of cucumber leaves inoculated with Pseudomonas syringae pv lachrymans (Psl) with those in non-inoculated systemic leaves, by examining the changes in the nicotinamide adenine dinucleotides pools, the concentration of soluble carbohydrates and activities/gene expression of carbohydrate metabolism-related enzymes, the expression of photosynthesis-related genes, and the tricarboxylic acid cycle-linked metabolite contents and enzyme activities. In the infected leaves, Psl induced a metabolic signature with an altered [NAD(P)H]/[NAD(P)+] ratio; decreased glucose and sucrose contents, along with a changed invertase gene expression; and increased glucose turnover and accumulation of raffinose, trehalose, and myo-inositol. The accumulation of oxaloacetic and malic acids, enhanced activities, and gene expression of fumarase and l-malate dehydrogenase, as well as the increased respiration rate in the infected leaves, indicated that Psl induced the tricarboxylic acid cycle. The changes in gene expression of ribulose-l,5-bis-phosphate carboxylase/oxygenase large unit, phosphoenolpyruvate carboxylase and chloroplast glyceraldehyde-3-phosphate dehydrogenase were compatible with a net photosynthesis decline described earlier. Psl triggered metabolic changes common to the infected and non-infected leaves, the dynamics of which differed quantitatively (e.g., malic acid content and metabolism, glucose-6-phosphate accumulation, and glucose-6-phosphate dehydrogenase activity) and those specifically related to the local or systemic response (e.g., changes in the sugar content and turnover). Therefore, metabolic changes in the systemic leaves may be part of the global effects of local infection on the whole-plant metabolism and also represent a specific acclimation response contributing to balancing growth and defense.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKornaś, Andrzej
dc.contributor.authorKuźniak-Gębarowska, Elżbieta
dc.contributor.authorKopczewski, Tomasz
dc.contributor.authorCiereszko, Iwona
dc.date.accessioned2024-01-24T16:28:23Z
dc.date.available2024-01-24T16:28:23Z
dc.date.copyright2022-10-17
dc.date.issued2022
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number20
dc.description.versionFINAL_PUBLISHED
dc.description.volume23
dc.identifier.doi10.3390/IJMS232012418
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/100505
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/23/20/12418/pdf
dc.languageeng
dc.pbn.affiliationeconomics and finance
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages12418
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enangular leaf spot disease
dc.subject.enmalic acid
dc.subject.enplant–pathogen interaction
dc.subject.enpyridine nucleotides
dc.subject.enraffinose
dc.subject.ensoluble sugars
dc.subject.enTCA cycle
dc.titleAlterations in Primary Carbon Metabolism in Cucumber Infected with Pseudomonas syringae pv lachrymans: Local and Systemic Responses
dc.typeJournalArticle
dspace.entity.typePublication