Artykuł w czasopiśmie
Brak miniatury
Licencja
CC-BYCC-BY - Uznanie autorstwa
 

A Functionalized Membrane Layer as Part of a Dressing to Aid Wound Healing

Uproszczony widok
dc.abstract.enPurpose: This study is an approach to a dressing platform based on support functionalized with oxygenating factors within an alginate layer, constituting a safe and even contact surface for interface with a wound. Methods: An alginate layer with incorporated oxygenating elements de- posited on the support patch was assessed. As an oxygenating factor, perfluorooctyl was applied, and the layer coatings in two options, cross-linked and not, were evaluated. The function of human dermal fibroblast cells cultured in the presence of these constructs was analyzed, as well as their morphology using flow cytometry, fluorescence microscopy, and scanning electron microscopy. In addition, the membrane coating material was assessed using FTIR, AFM, and SEM-EDX characteri- zation. Results: The applied membrane coatings adsorbed on the patch ensured the viability of the human fibroblasts cultured on the membranes during 10 days of culture. However, on the sixth day of culture, the percentage of live cells grown in the presence of cross-linked alginate with oxygenat-ing factor ((ALG-PFC)net) was significantly higher than that of the cells cultured in the presence of the alginate coatings alone. SEM-EDX analysis of the (ALG-PFC)net confirmed the presence of oxygenating and cross-linking factors. In addition, the regular granular branched structure of the layer coating material involving the oxygenating and cross-linking factors was observed using the AFM technique. Conclusion: The topography of the layer coating material involving the oxygenating and cross-linking factors ensures an even contact surface for interface with the wound. Considering 5-day intervals between dressing replacements, the platform with an oxygenating configuration ensuring the growth and morphology of the human fibroblasts can be recommended at this time as an element of a dressing system.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorStachowiak, Radosław
dc.contributor.authorGranicka, Ludomira
dc.contributor.authorDrabik, Monika
dc.contributor.authorKwiatkowska, Angelika
dc.contributor.authorLipko, Agata
dc.contributor.authorGRZECZKOWICZ, Anna
dc.contributor.authorMiłek, Tomasz
dc.contributor.authorGoliszewski, Jerzy
dc.contributor.authorStrawski, Marcin
dc.contributor.authorOleksinski, Leszek
dc.date.accessioned2024-01-24T17:37:40Z
dc.date.available2024-01-24T17:37:40Z
dc.date.copyright2022-09-27
dc.date.issued2022
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number10
dc.description.versionFINAL_PUBLISHED
dc.description.volume12
dc.identifier.doi10.3390/MEMBRANES12100936
dc.identifier.issn2077-0375
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/101556
dc.identifier.weblinkhttps://www.mdpi.com/2077-0375/12/10/936/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofMembranes
dc.relation.pages936
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enpolyelectrolyte membrane layer
dc.subject.endressing
dc.subject.enoxygenating factor
dc.subject.enpatch
dc.subject.enhuman fibroblasts
dc.titleA Functionalized Membrane Layer as Part of a Dressing to Aid Wound Healing
dc.typeJournalArticle
dspace.entity.typePublication