Journal Article
No Thumbnail Available
License
CC-BY-NC-NDCC-BY-NC-ND - Attribution-NonCommercial-NoDerivatives
 

Colorimetric point-of-care paper-based sensors for urinary creatinine with smartphone readout

Simple item page
cris.lastimport.scopus2024-02-12T20:28:32Z
dc.abstract.enCreatinine is a clinically significant analyte used to diagnose kidney condition. However, the literature still lacks in creatinine sensors fulfilling point-of-care testing requirements. In this paper, we have developed colorimetric paper-based creatinine sensors adhering to point-of-care testing principles. The signal readout is accomplished with a smartphone modified with 3D-printed elements and processed with a self-written application compromising computer vision algorithm for automatic detection of the colored zone. Two colorimetric methods – routinely used Jaff´ e method and an alternative one with 3,5-dinitrobenzoic acid were both tested and compared. Hue channel intensity from HSV color space and green channel from RGB color space was used as the analytical signal in Jaff´ e and 3,5-dinitrobenzoate method, respectively. For both kinds of sensors the linear range of the response covered the range significant for urinary analysis, with precision, expressed as RSD, below 5%. Limit of quantification for Jaff´ e method was 1.05 mmol⋅L− 1 whereas it was 0.82 mmol⋅L− 1 for 3,5-dinitrobenzoate method. The utility of the developed sensors to selectively quantify creatinine in undiluted urine was proved using artificial urine samples and the obtained recoveries were in the range from 70 to 129 %
dc.affiliationUniwersytet Warszawski
dc.contributor.authorSpeichert, Mikołaj
dc.contributor.authorGranica, Mateusz
dc.contributor.authorTymecki, Łukasz
dc.contributor.authorLewińska, Izabela
dc.date.accessioned2024-01-24T19:32:32Z
dc.date.available2024-01-24T19:32:32Z
dc.date.copyright2021-08-01
dc.date.issued2021
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.versionFINAL_PUBLISHED
dc.description.volume340
dc.identifier.doi10.1016/J.SNB.2021.129915
dc.identifier.issn0925-4005
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/103248
dc.identifier.weblinkhttps://api.elsevier.com/content/article/PII:S0925400521004846?httpAccept=text/xml
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofSensors and Actuators, B: Chemical
dc.relation.pages129915
dc.rightsCC-BY-NC-ND
dc.sciencecloudnosend
dc.subject.enCreatinine, Jaff´e, 3,5-dinitrobenzoic acid, Microfluidic paper-based device, Smartphone, Computer vision
dc.titleColorimetric point-of-care paper-based sensors for urinary creatinine with smartphone readout
dc.typeJournalArticle
dspace.entity.typePublication