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Characteristics of glucose oxidase immobilized on carbon-encapsulated iron nanoparticles decorated with polyethyleneimine

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cris.lastimport.scopus2024-02-12T20:28:56Z
dc.abstract.enThis study aimed to conjugate glucose oxidase (GOX) with carbon-encapsulated iron nanoparticles (Fe@C) which were functionalized with carboxylic groups and decorated with branched polyethyleneimine (PEI), in order to yield Fe@C-NH-PEI-GOX and Fe@C-(CH2)2-CONH-PEI-GOX derivatives. The process of electrostatic attachment of the GOX enzyme on the Fe@C-NH-PEI and Fe@C-(CH2)2CONH-PEI constructs was optimized, and the amount of enzyme attached on the constructs was also estimated. The steady-state kinetics of β-D-glucose oxidation by molecular oxygen, a reaction catalyzed by GOX, were compared using a native GOX enzyme obtained from Aspergillus niger and nanoparticles-conjugated GOX. The Fe@C-NH-PEI-GOX and Fe@C-(CH2)2-CONH-PEI-GOX constructs were formed by electrostatic interaction between positively charged Fe@C-NH-PEI and Fe@C-(CH2)2-CONH-PEI and negatively charged GOX in water media at pH 6. The activity of the immobilized enzyme on Fe@C-NH-PEI-GOX and Fe@C-(CH2)2-CONH-PEI-GOX derivatives was preserved, with Km values of 3271 and 1605 µM, respectively. However, the catalytic activity of Fe@C-NH-PEI-GOX and Fe@C-(CH2)2-CONH-PEI-GOX was reduced up to 20- and tenfold in comparison to that of the free GOX enzyme. The results of the study suggest that PEI is a promising cationic polymer with a high loading capacity and that the use of a simple adsorption process for preparing Fe@C-NH-PEI-GOX and Fe@C-(CH2)2-CONH-PEI-GOX conjugates is a highly efficient approach for stable immobilization of GOX enzyme on carbon-encapsulated iron nanoparticles.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorGrudzinski, Ireneusz P.
dc.contributor.authorSobczak, Kamil
dc.contributor.authorPoplawska, Magdalena
dc.contributor.authorBystrzejewski, Michał
dc.contributor.authorKasprzak, Artur
dc.contributor.authorBamburowicz-Klimkowska, Magdalena
dc.date.accessioned2024-01-24T19:06:37Z
dc.date.available2024-01-24T19:06:37Z
dc.date.issued2022
dc.description.financeNie dotyczy
dc.description.number2
dc.description.volume80
dc.identifier.doi10.1007/S00289-022-04125-1
dc.identifier.issn0170-0839
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/102890
dc.identifier.weblinkhttps://link.springer.com/content/pdf/10.1007/s00289-022-04125-1.pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofPolymer Bulletin
dc.relation.pages1565-1586
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enCarbon-encapsulated iron nanoparticles
dc.subject.enGlucose oxidase
dc.subject.enImmobilization processes
dc.subject.enPolyethyleneimine
dc.titleCharacteristics of glucose oxidase immobilized on carbon-encapsulated iron nanoparticles decorated with polyethyleneimine
dc.typeJournalArticle
dspace.entity.typePublication