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Inactivation of Genes Encoding MutL and MutS Proteins Influences Adhesion and Biofilm Formation by Neisseria gonorrhoeae

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dc.abstract.enNeisseria gonorrhoeae is an etiological agent of gonorrhea, which remains a global health problem. This bacterium possesses MutL and MutS DNA repair proteins encoded by mutL and mutS genes, whose inactivation causes a mutator phenotype. We have demonstrated the differential gene expression in N. gonorrhoeae mutL and mutS mutants using DNA microarrays. A subset of differentially expressed genes encodes proteins that can influence adhesion and biofilm formation. Compared to the wild-type strain, N. gonorrhoeae mutL and mutS mutants formed denser biofilms with increased biofilm-associated biomass on the abiotic surface. The N. gonorrhoeae mutS::km, but not the mutL mutant, was also more adherent and invasive to human epithelial cells. Further, during infection of epithelial cells with N. gonorrhoeae mutS::km, the expression of some bacterial genes encoding proteins that can influence gonococcal adhesion was changed compared with their expression in cells infected with the wild-type gonococcus, as well as of human genes’ encoding receptors utilized by N. gonorrhoeae (CD46, CEACAM 1, HSPG 2). Thus, deficiency in the mutS gene resulting in increased mutation frequency in singular organisms can be beneficial in populations because these mutants can be a source of features linked to microbial fitness.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorAdamczyk-Popławska, Monika
dc.contributor.authorLasek, Robert
dc.contributor.authorBącal, Paweł
dc.contributor.authorKwiatek, Agnieszka
dc.contributor.authorPłaczkiewicz, Jagoda
dc.date.accessioned2024-01-25T03:59:07Z
dc.date.available2024-01-25T03:59:07Z
dc.date.issued2019
dc.description.financeNie dotyczy
dc.description.number12
dc.description.volume7
dc.identifier.doi10.3390/MICROORGANISMS7120647
dc.identifier.issn2076-2607
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/109128
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofMicroorganisms
dc.relation.pages647
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enNeisseria gonorrhoeae
dc.subject.engene expression
dc.subject.enbiofilm
dc.subject.enadhesion
dc.subject.enMutL protein
dc.subject.enMutS protein
dc.titleInactivation of Genes Encoding MutL and MutS Proteins Influences Adhesion and Biofilm Formation by Neisseria gonorrhoeae
dc.typeJournalArticle
dspace.entity.typePublication