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Bacterial communities from the arsenic mine in Złoty Stok, Sudety mountains, Poland

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cris.lastimport.scopus2024-02-12T20:30:41Z
dc.abstract.enInvestigations of bacterial communities and characterization of mineralogy of the environment in the Złoty Stok As-Au deposit were carried out. PXRD analysis revealed the presence of picropharmacolite as the most common secondary arsenic mineral in the mine. Total DNA was extracted from slime streams or slime biofilms samples to investigate the bacterial communities. PCR amplification of 16S rDNA was performed followed by subcloning of its products. Over 170 clones were analyzed by means of RFLP method. Eight group of clones representing different restriction patterns were identified. The nucleotide sequences of their inserts suggest that bacteria present in the mine environment belong to: Flavobacteria, Sphingobacteriia, Bacteroides, Proteobacteria, Mollicutes and Firmicutes. The metagenomic approach allows to demonstrate a higher diversity of microbiota than classical microbiological studies of cultivable isolates.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorCłapa, Tomasz
dc.contributor.authorNarożna, Dorota
dc.contributor.authorSiuda, Rafał
dc.contributor.authorBorkowski, Andrzej
dc.contributor.authorSelwet, Marek
dc.contributor.authorMądrzak, Cezary
dc.contributor.authorKoźlecka, Ewa
dc.date.accessioned2024-01-24T18:14:44Z
dc.date.available2024-01-24T18:14:44Z
dc.date.copyright2017-09-27
dc.date.issued2017
dc.description.accesstimeBEFORE_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number3
dc.description.versionFINAL_AUTHOR
dc.description.volume66
dc.identifier.doi10.5604/01.3001.0010.4875
dc.identifier.issn1733-1331
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/101953
dc.languageeng
dc.pbn.affiliationearth and related environmental sciences
dc.relation.ispartofPolish Journal of Microbiology
dc.relation.pages375-381
dc.rightsCC-BY-NC-ND
dc.sciencecloudnosend
dc.subject.enarsenic mine ecosystem metagenomic approach microbial community supergene minerals
dc.titleBacterial communities from the arsenic mine in Złoty Stok, Sudety mountains, Poland
dc.typeJournalArticle
dspace.entity.typePublication