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Fluorometric method of creatinine determination employing 3,5-dinitrobenzoic acid

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dc.abstract.enA fluorometric method of creatinine determination was investigated. As a reagent 3,5-dinitrobenzoic acid is employed. The fluorometric 3,5-dinitrobenzoic acid-creatinine complex is formed in alkaline conditions with excitation and emission maxima at 410 nm and 480 nm, respectively. The conditions of the assays like solvent for 3,5-initrobenzoic acid, base concentration and reaction time were optimized. A linear calibration curve in the range from 10 to 500 μmol L–1 of creatinine was obtained. However, the substrate (3,5-dinitrobenzoic acid in organic solvent) is unstable, which limits application of proposed method.
dc.affiliationUniwersytet Warszawski
dc.conference.countryCzechy
dc.conference.datefinish2018-09-21
dc.conference.datestart2018-09-20
dc.conference.placePraga
dc.conference.seriesInternational Students Conference "Modern Analytical Chemistry"
dc.conference.seriesInternational Students Conference "Modern Analytical Chemistry"
dc.conference.seriesweblinkhttps://web.natur.cuni.cz/analchem/isc-mac/
dc.conference.shortcut14th International Students Conference Modern Analytical Chemistry
dc.conference.weblinkhttp://web.natur.cuni.cz/analchem/isc/isc14.pdf
dc.contributor.authorTymecki, Łukasz
dc.contributor.authorLewińska, Izabela
dc.contributor.authorMichalec, Michał
dc.date.accessioned2024-01-25T01:14:02Z
dc.date.available2024-01-25T01:14:02Z
dc.date.issued2018
dc.description.financeNie dotyczy
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/107311
dc.identifier.weblinkhttp://web.natur.cuni.cz/analchem/isc/isc14.pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.pages145-151
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.en35-dinitrobenzoic acid
dc.subject.encreatinine
dc.subject.enfluorimetry
dc.titleFluorometric method of creatinine determination employing 3,5-dinitrobenzoic acid
dc.typeJournalArticle
dspace.entity.typePublication