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Characterization of the virome of Paracoccus spp. (Alphaproteobacteria) by combined in silico and in vivo approaches.

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dc.abstract.enBacteria of the genus Paracoccus inhabit various pristine and anthropologically-shaped environments. Many Paracoccus spp. have biotechnological value and several are opportunistic human pathogens. Despite extensive knowledge of their metabolic potential and genome architecture, little is known about viruses of Paracoccus spp. So far, only three active phages infecting these bacteria have been identified. In this study, 16 Paracoccus strains were screened for the presence of active temperate phages, which resulted in the identification of five novel viruses. Mitomycin C-induced prophages were isolated, visualized and their genomes sequenced and thoroughly analyzed, including functional validation of their toxin-antitoxin systems. This led to the identification of the first active Myoviridae phage in Paracoccus spp. and four novel Siphoviridae phages. In addition, another 53 prophages were distinguished in silico within genomic sequences of Paracoccus spp. available in public databases. Thus, the Paracoccus virome was defined as being composed of 66 (pro)phages. Comparative analyses revealed the diversity and mosaicism of the (pro)phage genomes. Moreover, similarity networking analysis highlighted the uniqueness of Paracoccus (pro)phages among known bacterial viruses.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKozlowska, Patrycja
dc.contributor.authorRadlińska, Monika
dc.contributor.authorDziewit, Łukasz
dc.contributor.authorGolec, Piotr
dc.contributor.authorBartosik, Dariusz
dc.contributor.authorDecewicz, Przemysław
dc.date.accessioned2024-01-24T19:07:44Z
dc.date.available2024-01-24T19:07:44Z
dc.date.copyright2019-05-27
dc.date.issued2019
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.versionFINAL_PUBLISHED
dc.identifier.doi10.1038/S41598-019-44460-4
dc.identifier.issn2045-2322
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/102910
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofScientific Reports
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enParacoccus Alphaproteobacteria phage prophage toxin-antitoxin system DNA methyltransferase
dc.titleCharacterization of the virome of Paracoccus spp. (Alphaproteobacteria) by combined in silico and in vivo approaches.
dc.typeJournalArticle
dspace.entity.typePublication