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Dostęp zamkniętyPoint-of-care testing for C-reactive protein in a sequential microfluidic device
| cris.lastimport.scopus | 2024-02-12T20:25:57Z |
| dc.abstract.en | Point-of-care testing (POCT) devices are currently available for the detection of various important diseases owing to their easy-to-use, portable, and user-friendly nature. However, the multistep regent manipulation still impedes the performance of the device for end-users. To address this issue, we present a novel sequential flow-through microfluidic device that incorporates dual flow behaviors (fast-flow/delayed) into a single device for assessing the risk of cardiovascular disease (CVDs) using C-reactive protein (CRP) as the model analyte. The device features a fast-flow channel for automated washing of unbound CRP antigens and a delayed channel for flow of a redox reagent for electrochemical analysis. It requires only a single buffer loading, resulting in a total analysis time of 15 min. To overcome challenges related to storage stability and the affinity of antibodies in the manufacturing process, we integrated the P3-CRP peptide as a new bioreceptor for CRP detection. The performance of both the P3-CRP peptide and antibodies was assessed and examined using chronocoulometry. The proposed device exhibited a limit of detection at 47 pg mL−1 with a wide linear range (5 orders of magnitude). To validate its applicability, the device was tested with serum, plasma, and whole blood samples, yielding satisfactory results. |
| dc.affiliation | Uniwersytet Warszawski |
| dc.contributor.author | Jönsson-Niedziółka, Martin |
| dc.contributor.author | Pałys, Barbara |
| dc.contributor.author | Niedziółka-Jönsson, Joanna |
| dc.contributor.author | Szot-Karpińska, Katarzyna |
| dc.contributor.author | Boonkaew, Suchanat |
| dc.date.accessioned | 2024-01-25T17:00:07Z |
| dc.date.available | 2024-01-25T17:00:07Z |
| dc.date.issued | 2023 |
| dc.description.finance | Nie dotyczy |
| dc.description.volume | 397 |
| dc.identifier.doi | 10.1016/J.SNB.2023.134659 |
| dc.identifier.issn | 0925-4005 |
| dc.identifier.uri | https://repozytorium.uw.edu.pl//handle/item/116166 |
| dc.identifier.weblink | https://api.elsevier.com/content/article/PII:S0925400523013746?httpAccept=text/xml |
| dc.language | eng |
| dc.pbn.affiliation | chemical sciences |
| dc.relation.ispartof | Sensors and Actuators, B: Chemical |
| dc.relation.pages | 134659 |
| dc.rights | ClosedAccess |
| dc.sciencecloud | nosend |
| dc.subject.en | C-reactive protein |
| dc.subject.en | Point-of-care testing |
| dc.subject.en | Peptide |
| dc.subject.en | Capillary-driven microfluidic device |
| dc.subject.en | Label-free immunoassay |
| dc.subject.en | Electrochemical detection |
| dc.title | Point-of-care testing for C-reactive protein in a sequential microfluidic device |
| dc.type | JournalArticle |
| dspace.entity.type | Publication |