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Yeast pentatricopeptide protein Dmr1 (Ccm1) binds a repetitive AU-rich motif in the small subunit mitochondrial ribosomal RNA

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cris.lastimport.scopus2024-02-12T20:15:27Z
dc.abstract.enPPR proteins are a diverse family of RNA binding factors found in all Eukaryotic lineages. They perform multiple functions in the expression of organellar genes, mostly on the post-transcriptional level. PPR proteins are also significant determinants of evolutionary nucleo-organellar compatibility. Plant PPR proteins recognize their RNA substrates using a simple modular code. No target sequences recognized by animal or yeast PPR proteins were identified prior to the present study, making it impossible to assess whether this plant PPR code is conserved in other organisms. Dmr1p (Ccm1p, Ygr150cp) is a S. cer- evisiae PPR protein essential for mitochondrial gene expression and involved in the stability of 15S ribosomal RNA. We demonstrate that in vitro Dmr1p specifically binds a motif composed of multiple AUA repeats occurring twice in the 15S rRNA sequence as the minimal 14 nt (AUA)4AU or longer (AUA)7 variant. Short RNA fragments containing this motif are protected by Dmr1p from exoribonucleolytic activity in vitro. Presence of the identified motif in mtDNA of different yeast species correlates with the compatibility between their Dmr1p orthologs and S. cerevisiae mtDNA. RNA recognition by Dmr1p is likely based on a rudimentary form of a PPR code specifying U at every third position, and depends on other factors, like RNA structure.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorPiątkowski, Jakub
dc.contributor.authorGolik, Paweł
dc.date.accessioned2024-01-26T12:17:49Z
dc.date.available2024-01-26T12:17:49Z
dc.date.copyright2020-08-17
dc.date.issued2020
dc.description.accesstimeBEFORE_PUBLICATION
dc.description.financeŚrodki finansowe przyznane na realizację projektu w zakresie badań naukowych lub prac rozwojowych
dc.description.versionFINAL_PUBLISHED
dc.description.volume26
dc.identifier.doi10.1261/RNA.074880.120
dc.identifier.issn1355-8382
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/125644
dc.identifier.weblinkhttp://dx.doi.org/10.1261/rna.074880.120
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofRNA
dc.relation.pages1268-1282
dc.rightsCC-BY
dc.sciencecloudnosend
dc.titleYeast pentatricopeptide protein Dmr1 (Ccm1) binds a repetitive AU-rich motif in the small subunit mitochondrial ribosomal RNA
dc.typeJournalArticle
dspace.entity.typePublication