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Quantitative 3-D morphometric analysis of individual dendritic spines

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cris.lastimport.scopus2024-02-12T20:04:01Z
dc.abstract.enThe observation and analysis of dendritic spines morphological changes poses a major challenge in neuroscience studies. The alterations of their density and/or morphology are indicators of the cellular processes involved in neural plasticity underlying learning and memory, and are symptomatic in neuropsychiatric disorders. Despite ongoing intense investigations in imaging approaches, the relationship between changes in spine morphology and synaptic function is still unknown. The existing quantitative analyses are difficult to perform and require extensive user intervention. Here, we propose a new method for (1) the three-dimensional (3-D) segmentation of dendritic spines using a multi-scale opening approach and (2) define 3-D morphological attributes of individual spines for the effective assessment of their structural plasticity. The method was validated using confocal light microscopy images of dendritic spines from dissociated hippocampal cultures and brain slices (1) to evaluate accuracy relative to manually labeled ground-truth annotations and relative to the state-of-the-art Imaris tool, (2) to analyze reproducibility of user-independence of the segmentation method, and (3) to quantitatively analyze morphological changes in individual spines before and after chemically induced long-term potentiation. The method was monitored and used to precisely describe the morphology of individual spines in real-time using consecutive images of the same dendritic fragment.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorWlodarczyk, Jakub
dc.contributor.authorDas, Nirmal
dc.contributor.authorBaczynska, Ewa
dc.contributor.authorBasu, Subhadip
dc.contributor.authorPlewczyński, Dariusz
dc.contributor.authorSaha, Punam Kumar
dc.contributor.authorMagnowska, Marta
dc.contributor.authorROSZKOWSKA, MATYLDA
dc.date.accessioned2024-01-25T18:43:44Z
dc.date.available2024-01-25T18:43:44Z
dc.date.issued2018
dc.description.financeNie dotyczy
dc.identifier.doi10.1038/S41598-018-21753-8
dc.identifier.issn2045-2322
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/117680
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofScientific Reports
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.en2018 Plewczyński
dc.titleQuantitative 3-D morphometric analysis of individual dendritic spines
dc.typeJournalArticle
dspace.entity.typePublication