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Multicommutated Flow Analysis System for Determination of Horseradish Peroxidase and Its Inhibitors

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cris.lastimport.scopus2024-02-12T20:18:51Z
dc.abstract.enA fully mechanized multicommutated flow analysis (MCFA) system dedicated to deter-mining horseradish peroxidase (HRP) activity was developed. Detection was conducted using a flow-through optoelectronic detector-constructed of paired LEDs operating according to the paired emitter-detector diode (PEDD) principle. The PEDD-MCFA system is dedicated to monitoring the enzyme-catalyzed oxidation of p-phenylenediamine (pPD) by a hydrogen peroxide. Under optimized conditions, the presented bioanalytical system was characterized by a linear response range (33.47–200 U/L) with a detection limit at 10.54 U/L HRP activity and 1.66 mV·L/U sensitivity, relatively high throughput (12 signals recordings per hour), and acceptable precision (RSD below 6%). Additionally, the utility of the developed PEDD-MCFA system for the determination of HRP inhibitors allowing the detection of selected thiols at micromolar levels, is demonstrated. The practical utility of the flow system was illustrated by the analysis of some dietary supplements containing L-cysteine, N-acetylcysteine, and L-glutathione.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorGłowacka, Justyna
dc.contributor.authorStrzelak, Kamil
dc.contributor.authorKoncki, Robert
dc.date.accessioned2024-01-25T13:05:57Z
dc.date.available2024-01-25T13:05:57Z
dc.date.copyright2021-09-16
dc.date.issued2021
dc.description.accesstimeAT_PUBLICATION
dc.description.financeŚrodki finansowe, o których mowa w art. 365 pkt. 2 ustawy
dc.description.number18
dc.description.versionFINAL_PUBLISHED
dc.description.volume26
dc.identifier.doi10.3390/MOLECULES26185630
dc.identifier.issn1420-3049
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/113054
dc.identifier.weblinkhttps://www.mdpi.com/1420-3049/26/18/5630/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofMolecules
dc.relation.pages5630
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enFlow analysis
dc.subject.enHorseradish peroxidase
dc.subject.enMulticommutation
dc.subject.enThiols
dc.titleMulticommutated Flow Analysis System for Determination of Horseradish Peroxidase and Its Inhibitors
dc.typeJournalArticle
dspace.entity.typePublication