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Two SnRK2-Interacting Calcium Sensor Isoforms Negatively Regulate SnRK2 Activity by Different Mechanisms.

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cris.lastimport.scopus2024-02-12T20:15:37Z
dc.abstract.enSNF1-related protein kinases 2 (SnRK2s) are key signaling elements regulating abscisic acid-dependent plant development and responses to environmental stresses. Our previous data showed that the SnRK2-interacting Calcium Sensor (SCS) inhibits SnRK2 activity. Use of alternative transcription start sites located within the Arabidopsis (Arabidopsis thaliana) AtSCS gene results in two in-frame transcripts and subsequently two proteins, that differ only by the sequence position of the N terminus. We previously described the longer AtSCS-A, and now describe the shorter AtSCS-B and compare the two isoforms. The two isoforms differ substantially in their expression profiles in plant organs and in response to environmental stresses, in their calcium binding properties, and in their conformational dynamics in the presence and absence of Ca2+. Only AtSCS-A has the features of a calcium sensor. Both forms inhibit SnRK2 activity, but while AtSCS-A requires calcium for inhibition, AtSCS-B does not. Analysis of Arabidopsis plants stably expressing 35S::AtSCS-A-c-myc or 35S::AtSCS-B-c-myc in the scs-1 knockout mutant background revealed that, in planta, both forms are negative regulators of abscisic acid-induced SnRK2 activity and regulate plant resistance against water deficit. Moreover, the data highlight biochemical, biophysical, and functional properties of EF-hand-like motifs in plant proteins.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorDadlez, Michał
dc.contributor.authorPoznański, Jarosław
dc.contributor.authorKlimecka, Maria
dc.contributor.authorDobrowolska, Grażyna
dc.contributor.authorEngh, Richard A.
dc.contributor.authorTarnowski, Krzysztof
dc.contributor.authorBucholc, Maria
dc.contributor.authorLichocka, Małgorzata
dc.contributor.authorKulik, Anna
dc.contributor.authorGoch, Grażyna
dc.contributor.authorFedak, Halina
dc.contributor.authorCiesielski, Arkadiusz
dc.contributor.authorPierechod, Marcin
dc.date.accessioned2024-01-26T11:09:13Z
dc.date.available2024-01-26T11:09:13Z
dc.date.copyright2019-11-07
dc.date.issued2020
dc.description.accesstimeBEFORE_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number2
dc.description.versionORIGINAL_AUTHOR
dc.description.volume182
dc.identifier.doi10.1104/PP.19.00900
dc.identifier.issn0032-0889
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/123976
dc.identifier.weblinkhttp://www.plantphysiol.org/content/182/2/1142
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofPlant Physiology
dc.relation.pages1142-1160
dc.rightsOther
dc.sciencecloudnosend
dc.subject.enAGROBACTERIUM-MEDIATED TRANSFORMATION
dc.subject.enACTIVATED PROTEIN-KINASES
dc.subject.enABSCISIC-ACID
dc.subject.enSIGNALING NETWORK
dc.subject.enGENE-EXPRESSION
dc.subject.enANION CHANNEL
dc.subject.enFLORAL DIP
dc.subject.enSTRESS
dc.subject.enRESPONSES
dc.subject.enOST1
dc.titleTwo SnRK2-Interacting Calcium Sensor Isoforms Negatively Regulate SnRK2 Activity by Different Mechanisms.
dc.typeJournalArticle
dspace.entity.typePublication