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Assessment of Bacterial Contamination of Air at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland): Selection of an Optimal Growth Medium for Analyzing Airborne Bacteria Diversity

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cris.lastimport.scopus2024-02-12T20:03:40Z
dc.abstract.enThere is no standardized protocol for the assessment of microbial air contamination in museums and other cultural heritage sites. Therefore, most museums conduct such assessments based on their own guidelines or good practices. Usually, microbial air contamination is assessed using only classical microbiology methods with the application of a single growth medium. Therefore, this medium should be carefully selected to limit any selective cultivation bias. Metabarcoding, i.e., a next-generation sequencing (NGS)-based method, combined with classical microbiological culturing was used to assess the effectiveness of various media applications in microbiological screening at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland). The obtained results indicated that when using a classical microbiology approach to assess the microbial air contamination at the museum, the selection of a proper growth medium was critical. It was shown that the use of rich media (commonly applied by museum conservators) introduced significant bias by severely underreporting putative human pathogens and the bacterial species involved in biodeterioration. Therefore, we recommend the use of other media, such as Frazier or Reasoner’s 2A (R2A) medium, as they could yield more diverse communities and recovered the highest number of genera containing human pathogens, which may be suitable for public health assessments.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorLaudy, Agnieszka
dc.contributor.authorDziewit, Łukasz
dc.contributor.authorCiuchcinski, Karol
dc.contributor.authorDyda, Magdalena
dc.contributor.authorDrabik, Paulina
dc.contributor.authorDziurzyński, Mikołaj
dc.contributor.authorSzych, Anna
dc.date.accessioned2024-01-24T16:57:00Z
dc.date.available2024-01-24T16:57:00Z
dc.date.copyright2020-10-13
dc.date.issued2020
dc.description.accesstimeAT_PUBLICATION
dc.description.financeInne
dc.description.number20
dc.description.versionFINAL_PUBLISHED
dc.description.volume10
dc.identifier.doi10.3390/APP10207128
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/101206
dc.identifier.weblinkhttps://doi.org/10.3390/app10207128
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofApplied Sciences (Switzerland)
dc.relation.pages7128
dc.rightsCC-BY
dc.sciencecloudnosend
dc.titleAssessment of Bacterial Contamination of Air at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland): Selection of an Optimal Growth Medium for Analyzing Airborne Bacteria Diversity
dc.typeJournalArticle
dspace.entity.typePublication