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Photometric flow system for the determination of serum lactate dehydrogenase activity

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cris.lastimport.scopus2024-02-12T20:04:59Z
dc.abstract.enThe routine method for LDH (Lactate dehydrogenase) activity determination is to monitor the increase of NADH concentration at 340 nm. There are some inconvenience in taking measurements in the near-UV region, especially in the case of serum samples analysis. In this work, two modifications of the routine LDH activity assay based on the use of reducing properties of NADH have been compared. Both methods involved the reduction of compounds that can be easily determined by well-known methods, ferric ion (with ferrozine) and nitrotetrazolium blue (NBT). A fully-mechanized Multicommutated Flow Analysis-Paired Emitter Detector Diode (MCFA-PEDD) system based on solenoid devices was developed and applied for both methods. The linear ranges obtained for Fe-ferrozine and NBT methods are 6.0–200.0 U L−1 and 10.0–250.0 U L−1 with estimated detection limits at 0.2 U L−1 and 4.5 U L−1, respectively. The low LOQ values enabled 10-fold sample dilutions, which is advantageous for samples with limited available volume. The Fe-ferrozine method is more selective for LDH activity in the presence of glucose, ascorbic acid, albumin, bilirubin, copper and calcium ions than NBT method. To confirm the analytical usefulness of the proposed flow system, the analysis of real human serum samples was carried out. The statistic tests showed satisfactory correlation between the results obtained for both developed methods and those received using the reference method.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorStrzelak, Kamil
dc.contributor.authorKoncki, Robert
dc.contributor.authorWiśniewska, Agnieszka
dc.contributor.authorGłowacka, Justyna
dc.date.accessioned2024-01-25T16:36:49Z
dc.date.available2024-01-25T16:36:49Z
dc.date.copyright2023-06-23
dc.date.issued2023
dc.description.accesstimeAT_PUBLICATION
dc.description.financeŚrodki finansowe, o których mowa w art. 365 pkt. 2 ustawy
dc.description.versionFINAL_PUBLISHED
dc.description.volume265
dc.identifier.doi10.1016/J.TALANTA.2023.124817
dc.identifier.issn0039-9140
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/115846
dc.identifier.weblinkhttps://api.elsevier.com/content/article/PII:S0039914023005684?httpAccept=text/xml
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofTalanta
dc.relation.pages124817
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enFerrozine
dc.subject.enFlow analysis
dc.subject.enLactate dehydrogenase
dc.subject.enMulticommutation
dc.subject.enNitrotetrazolium blue
dc.titlePhotometric flow system for the determination of serum lactate dehydrogenase activity
dc.typeJournalArticle
dspace.entity.typePublication