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Valine/isoleucine variants drive selective pressure in the VP1 sequence of EV-A71 enteroviruses

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dc.abstract.enBackground: In 2011–2012, Northern Vietnam experienced its first large scale hand foot and mouth disease (HFMD) epidemic. In 2011, a major HFMD epidemic was also reported in South Vietnam with fatal cases. This 2011–2012 outbreak was the first one to occur in North Vietnam providing grounds to study the etiology, origin and dynamic of the disease. We report here the analysis of the VP1 gene of strains isolated throughout North Vietnam during the 2011–2012 outbreak and before. Methods: The VP1 gene of 106 EV-A71 isolates from North Vietnam and 2 from Central Vietnam were sequenced. Sequence alignments were analyzed at the nucleic acid and protein level. Gene polymorphism was also analyzed. A Factorial Correspondence Analysis was performed to correlate amino acid mutations with clinical parameters. Results: The sequences were distributed into four phylogenetic clusters. Three clusters corresponded to the subgenogroup C4 and the last one corresponded to the subgenogroup C5. Each cluster displayed different polymorphism characteristics. Proteins were highly conserved but three sites bearing only Isoleucine (I) or Valine (V) were characterized. The isoleucine/valine variability matched the clusters. Spatiotemporal analysis of the I/V variants showed that all variants which emerged in 2011 and then in 2012 were not the same but were all present in the region prior to the 2011–2012 outbreak. Some correlation was found between certain I/V variants and ethnicity and severity. Conclusions: The 2011–2012 outbreak was not caused by an exogenous strain coming from South Vietnam or elsewhere but by strains already present and circulating at low level in North Vietnam. However, what triggered the outbreak remains unclear. A selective pressure is applied on I/V variants which matches the genetic clusters. I/V variants were shown on other viruses to correlate with pathogenicity. This should be investigated in EV-A71. I/V variants are an easy and efficient way to survey and identify circulating EV-A71 strains. Keywords: VP1, HFMD, Enterovirus, EV-A71, Vietnam
dc.affiliationUniwersytet Warszawski
dc.contributor.authorHou, Yan’An
dc.contributor.authorChua, Robert
dc.contributor.authorKister, Guilhem
dc.contributor.authorMoulia, Catherine
dc.contributor.authorRave, Patrice
dc.contributor.authorAfelt, Aneta
dc.contributor.authorHuong, Le Thi Song
dc.contributor.authorDwivedi, Ankit
dc.contributor.authorSessions, October Michael
dc.contributor.authorDuy, Nghia Ngu
dc.contributor.authorHuong, Le Thi Thanh
dc.contributor.authorFrutos, Roger
dc.contributor.authorHien, Nguyen Tran
dc.contributor.authorCornillot, Emmanuel
dc.contributor.authorGavotte, Laurent
dc.contributor.authorThanh, Nguyen Thi Hien
dc.contributor.authorDevaux, Christian
dc.contributor.authorDuong, Tran Nhu
dc.contributor.authorGubler, Duane J.
dc.contributor.authorThiem, Vu Dinh
dc.date.accessioned2024-01-26T11:45:16Z
dc.date.available2024-01-26T11:45:16Z
dc.date.copyright2017-05-08
dc.date.issued2017
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.versionFINAL_PUBLISHED
dc.identifier.doi10.1186/S12879-017-2427-4
dc.identifier.issn1471-2334
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/124512
dc.identifier.weblinkhttps://doi.org/10.1186/s12879-017-2427-4
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofBMC Infectious Diseases
dc.relation.pages333
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enVP1
dc.subject.enHFMD
dc.subject.enEnterovirus
dc.subject.enEV-A71
dc.subject.enVietnam
dc.titleValine/isoleucine variants drive selective pressure in the VP1 sequence of EV-A71 enteroviruses
dc.typeJournalArticle
dspace.entity.typePublication