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Photocyclic behavior of rhodopsin induced by a novel isomerization mechanism

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cris.lastimport.scopus2024-02-12T20:21:29Z
dc.abstract.enVertebrate rhodopsin (Rh) contains 11-cis-retinal as a chromophore to convert light energy into visual signals. On absorption of light, 11-cis-retinal is isomerized to all- trans-retinal, constituting a one-way reaction that activates transducin (Gt) followed by chromo- phore release. Here we report that bovine Rh, regenerated instead with a six-carbon-ring retinal chromophore featuring a C11=C12 double bond locked in its cis conformation (Rh6mr), employs an atypical isomerization mechanism by converting 11-cis to an 11,13-dicis configuration for prolonged Gt activation. Time-dependent UV-vis spectroscopy, HPLC, and molecular mechanics analyses revealed an atypical thermal reisomerization of the 11,13-dicis to the 11-cis configuration on a slow timescale, which enables Rh6mr to function in a photocyclic manner similar to that of microbial Rhs. With this photocyclic behavior, Rh6mr repeatedly recruits and ac-tivates Gt in response to light stimuli, making it an excellent can-didate for optogenetic tools based on retinal analog-bound vertebrate Rhs. Overall, these comprehensive structure–function studies unveil a unique photocyclic mechanism of Rh activation by an 11-cis–to–11,13-dicis isomerization.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorGao, Songqi
dc.contributor.authorFilipek, Sławomir
dc.contributor.authorJastrzebska, Beata
dc.contributor.authorGulati, Sahil
dc.contributor.authorPlaceres, Ángel
dc.contributor.authorBanerjee, Surajit
dc.contributor.authorGunderson, Karl
dc.contributor.authorMiszta, Przemysław
dc.contributor.authorKatayama, Kota
dc.contributor.authorTochtrop, Gregory
dc.contributor.authorMogi, Muneto
dc.contributor.authorKiser, Philip
dc.contributor.authorPalczewski, Krzysztof
dc.contributor.authorStewart, Phoebe
dc.date.accessioned2024-01-25T16:36:27Z
dc.date.available2024-01-25T16:36:27Z
dc.date.issued2017
dc.description.financeNie dotyczy
dc.description.number13
dc.description.volume114
dc.identifier.doi10.1073/PNAS.1617446114
dc.identifier.issn0027-8424
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/115825
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America
dc.relation.pagesE2608-E2615
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enrhodopsin
dc.subject.envision
dc.subject.enGPCR
dc.subject.enisomerization
dc.subject.enchromophore
dc.titlePhotocyclic behavior of rhodopsin induced by a novel isomerization mechanism
dc.typeJournalArticle
dspace.entity.typePublication