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Suppression of External NADPH Dehydrogenase—NDB1 in Arabidopsis thaliana Confers Improved Tolerance to Ammonium Toxicity via Efficient Glutathione/Redox Metabolism

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cris.lastimport.scopus2024-02-12T20:11:03Z
dc.abstract.enEnvironmental stresses, including ammonium (NH4+) nourishment, can damage key mitochondrial components through the production of surplus reactive oxygen species (ROS) in the mitochondrial electron transport chain. However, alternative electron pathways are significant for efficient reductant dissipation in mitochondria during ammonium nutrition. The aim of this study was to define the role of external NADPH-dehydrogenase (NDB1) during oxidative metabolism of NH4+-fed plants. Most plant species grown with NH4+ as the sole nitrogen source experience a condition known as “ammonium toxicity syndrome”. Surprisingly, transgenic Arabidopsis thaliana plants suppressing NDB1 were more resistant to NH4+ treatment. The NDB1 knock-down line was characterized by milder oxidative stress symptoms in plant tissues when supplied with NH4+. Mitochondrial ROS accumulation, in particular, was attenuated in the NDB1 knock-down plants during NH4+ treatment. Enhanced antioxidant defense, primarily concerning the glutathione pool, may prevent ROS accumulation in NH4+-grown NDB1-suppressing plants. We found that induction of glutathione peroxidase-like enzymes and peroxiredoxins in the NDB1-surpressing line contributed to lower ammonium-toxicity stress. The major conclusion of this study was that NDB1 suppression in plants confers tolerance to changes in redox homeostasis that occur in response to prolonged ammonium nutrition, causing cross tolerance among plants.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorBurian, Maria
dc.contributor.authorRasmusson, Allan G
dc.contributor.authorSzal, Bożena
dc.contributor.authorPodgórska, Anna
dc.contributor.authorTarnowska, Agata
dc.contributor.authorJakubiak, Monika
dc.contributor.authorOstaszewska-Bugajska, Monika
dc.contributor.authorBorysiuk, Klaudia
dc.date.accessioned2024-01-26T09:24:48Z
dc.date.available2024-01-26T09:24:48Z
dc.date.copyright2018-05-09
dc.date.issued2018
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.versionFINAL_PUBLISHED
dc.description.volume19
dc.identifier.doi10.3390/IJMS19051412
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/121158
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/19/5/1412
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages1412
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enammonium toxicity
dc.subject.enexternal type II NADPH dehydrogenase
dc.subject.englutathione metabolism
dc.subject.enreactive oxygen species
dc.subject.enredox homeostasis
dc.titleSuppression of External NADPH Dehydrogenase—NDB1 in Arabidopsis thaliana Confers Improved Tolerance to Ammonium Toxicity via Efficient Glutathione/Redox Metabolism
dc.typeJournalArticle
dspace.entity.typePublication