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Identification of proteins, drying oils, waxes and resins in the works of art micro-samples by chromatographic and mass spectrometric techniques

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dc.abstract.enSimplified method for simultaneous identification of proteins, drying oils, waxes, and resins in the works-of-art samples was developed. Liquid chromatography with mass spectrometry and gas chromatography with mass spectrometry were used to identify natural materials most frequently encountered in historical paintings. Protein binders were extracted with ammonia and purified using miniaturized solid-phase microextraction (Omix tips) to efficiently suppress matrix interferences. Zwitterionic stationary phase was used for separation of 16 underivatized amino acids analysis with hydrophilic interaction liquid chromatography that was subsequently quantified with liquid chromatography with mass spectrometry. Gas chromatography with mass spectrometry was used to analyze drying oils, waxes, and resins after one-step saponification/transmethylation with (m-trifluoromethylphenyl)trimethylammonium hydroxide (Meth-Prep II). While the drawback of this reagent is low reactivity towards hydroxyl groups, sample pretreatment was much simpler as compared to the other methods. Fatty acids derivatization with the Meth-Prep II reagent was compared with their silylation using N,O-bis(trimethylsilyl) trifluoroacetamide/trimethylchlorosilane mixture. It was concluded that fatty acids analysis as their methyl esters instead of trimethylsilyl esters had a minor impact on the method sensitivity. The developed method was used to analyze samples from 16th and 17th century historical paintings.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorLaudy, Agnieszka
dc.contributor.authorGaneczko, Monika
dc.contributor.authorBiesaga, Magdalena
dc.contributor.authorGierczak, Tomasz
dc.contributor.authorWitkowski, Bartłomiej
dc.contributor.authorDuchnowicz, Anna
dc.date.accessioned2024-01-25T03:43:34Z
dc.date.available2024-01-25T03:43:34Z
dc.date.issued2018
dc.description.financeNie dotyczy
dc.description.number3
dc.description.volume41
dc.identifier.doi10.1002/JSSC.201700937
dc.identifier.issn1615-9306
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/108880
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofJournal of Separation Science
dc.relation.pages630-638
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enbinding media
dc.subject.enchromatography
dc.subject.enmass spectrometry
dc.subject.enpaint
dc.subject.enworks of art
dc.titleIdentification of proteins, drying oils, waxes and resins in the works of art micro-samples by chromatographic and mass spectrometric techniques
dc.typeJournalArticle
dspace.entity.typePublication