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Genome Study of a Novel Virulent Phage vB_SspS_KASIA and Mu-like Prophages of Shewanella sp. M16 Provides Insights into the Genetic Diversity of the Shewanella Virome

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cris.lastimport.scopus2024-02-12T20:15:12Z
dc.abstract.enShewanella is a ubiquitous bacterial genus of aquatic ecosystems, and its bacteriophages are also isolated from aquatic environments (oceans, lakes, ice, and wastewater). In this study, the isolation and characterization of a novel virulent Shewanella phage vB_SspS_KASIA and the identification of three prophages of its host, Shewanella sp. M16, including a mitomycin-inducible Mu-like siphovirus, vB_SspS_MuM16-1, became the starting point for comparative analyses of phages infecting Shewanella spp. and the determination of their position among the known bacterial viruses. A similarity networking analysis revealed the high diversity of Shewanella phages in general, with vB_SspS_KASIA clustering exclusively with Colwellia phage 9A, with which it forms a single viral cluster composed of two separate viral subclusters. Furthermore, vB_SspS_MuM16-1 presented itself as being significantly different from the phages deposited in public databases, expanding the diversity of the known Mu-like phages and giving potential molecular markers for the identification of Mu-like prophages in bacterial genomes. Moreover, the functional analysis performed for vB_SspS_KASIA suggested that, despite the KASIA host, the M16 strain grows better in a rich medium and at 30 °C the phage replication cycle seems to be optimal in restrictive culture conditions mimicking their natural environment, the Zloty Stok gold and arsenic mine.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorBujak, Katarzyna
dc.contributor.authorDecewicz, Przemysław
dc.contributor.authorRadlińska, Monika
dc.contributor.authorRosinska, Joanna M.
dc.date.accessioned2024-01-25T02:05:36Z
dc.date.available2024-01-25T02:05:36Z
dc.date.copyright2021-10-14
dc.date.issued2021
dc.description.accesstimeBEFORE_PUBLICATION
dc.description.financeŚrodki finansowe przyznane na realizację projektu w zakresie badań naukowych lub prac rozwojowych
dc.description.number20
dc.description.versionFINAL_PUBLISHED
dc.description.volume22
dc.identifier.doi10.3390/IJMS222011070
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/107845
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/22/20/11070/pdf
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages11070
dc.rightsCC-BY
dc.sciencecloudnosend
dc.titleGenome Study of a Novel Virulent Phage vB_SspS_KASIA and Mu-like Prophages of Shewanella sp. M16 Provides Insights into the Genetic Diversity of the Shewanella Virome
dc.typeJournalArticle
dspace.entity.typePublication