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Exploring the genome of Arctic Psychrobacter sp. DAB_AL32B and construction of novel Psychrobacter-specific cloning vectors of an increased carrying capacity

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cris.lastimport.scopus2024-02-12T20:14:31Z
dc.abstract.enCold-active bacteria are currently of great interest in biotechnology, and their genomic and physiological features have been extensively studied. One of the model psychrotolerant bacteria are Psychrobacter spp. Analysis of Arctic psychrophilic Psychrobacter sp. DAB_AL32B genome content provided an insight into its overall stress response, and genes conferring protection against various life-limiting factors (i.e., low temperature, increased ultraviolet radiation, oxidative stress and osmotic pressure) were recognized and described. Moreover, it was revealed that the strain carries a large plasmid pP32BP2. Its replication system was used for the construction of two novel shuttle vectors (pPS-NR—Psychrobacter-Escherichia coli-specific plasmid and pPS-BR—Psychrobacter-various Proteobacteria-specific plasmid) of an increased carrying capacity, which may be used for genetic engineering of Psychrobacter spp.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorCiok, Anna
dc.contributor.authorDziewit, Łukasz
dc.date.accessioned2024-01-25T00:10:16Z
dc.date.available2024-01-25T00:10:16Z
dc.date.issued2019
dc.description.financeNie dotyczy
dc.description.volume201
dc.identifier.doi10.1007/S00203-018-1595-Y
dc.identifier.issn0302-8933
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/106821
dc.identifier.weblinkhttps://link.springer.com/article/10.1007/s00203-018-1595-y
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofArchives of Microbiology
dc.relation.pages559-569
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enPsychrobacter sp. DAB_AL32B
dc.subject.enPsychrobacter-specific vector
dc.subject.enDraft genome sequence
dc.subject.enStress adaptation
dc.titleExploring the genome of Arctic Psychrobacter sp. DAB_AL32B and construction of novel Psychrobacter-specific cloning vectors of an increased carrying capacity
dc.typeJournalArticle
dspace.entity.typePublication