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Targeting and killing the Ever-Challenging ulcer bug

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cris.lastimport.scopus2024-02-12T20:22:28Z
dc.abstract.enTreating Helicobacter pylori (H. pylori) infections has been a never-ending challenge, which has contributed to the high incidence of gastric cancer. The antibiotics commonly used are not reaching the infection site in its active state and in a concentration high enough to effectively kill the bacteria. In this context, amoxicillin-loaded lipid nanoparticles with carefully chosen materials were developed, namely dioleoylphosphatidylethanolamine (DOPE) as a targeting agent and Tween®80 and linolenic acid as antimicrobial agents. This work shows the ability of these nanoparticles in (i) targeting the bacteria (imaging flow cytometry) and inhibiting their adhesion to MKN-74 cells (bacteria-gastric cells adhesion model); (ii) killing the bacteria even as an antibiotic-free strategy (time-kill kinetic studies, scanning electron microscopy, and bacterial membrane permeability studies); (iii) overcoming gastrointestinal features using a newly developed in vitro infection model that includes both physical (epithelial cells and mucus) and the chemical (acid medium) barriers; and in (iv) being incorporated in a floating system that can increase the retention time at the stomach. Overall, this work presents an effective nanosystem to deal with the ulcer-bug. Besides, it also provides two innovative tools transferable to other fields – an in vitro infection model and a floating system to incorporate nanoparticles
dc.affiliationUniwersytet Warszawski
dc.contributor.authorSłowiński, Mateusz
dc.contributor.authorLopes-de-Campos, Daniela
dc.contributor.authorSeabra, Catarina Leal
dc.contributor.authorPinto, Rita M.
dc.contributor.authorNunes, Cláudia
dc.contributor.authorMartins, M. Cristina L.
dc.contributor.authorReis, Salette
dc.contributor.authorSarmento, Bruno
dc.date.accessioned2024-01-26T09:41:26Z
dc.date.available2024-01-26T09:41:26Z
dc.date.issued2022
dc.description.financeNie dotyczy
dc.description.volume617
dc.identifier.doi10.1016/J.IJPHARM.2022.121582
dc.identifier.issn0378-5173
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/121553
dc.identifier.weblinkhttp://dx.doi.org/10.1016/j.ijpharm.2022.121582
dc.languageeng
dc.relation.ispartofInternational Journal of Pharmaceutics
dc.relation.pagesart.no. 121582
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enFloating system
dc.subject.enHelicobacter pylori
dc.subject.enInfection model
dc.subject.enLipid nanoparticles
dc.subject.enTargeting
dc.titleTargeting and killing the Ever-Challenging ulcer bug
dc.typeJournalArticle
dspace.entity.typePublication