Artykuł w czasopiśmie
Brak miniatury
Licencja
CC-BY-NC-SACC-BY-NC-SA - Uznanie autorstwa - Użycie niekomercyjne - Na tych samych warunkach
 

Selected ALKBH dioxygenases are overexpressed in salivary gland tumours

Uproszczony widok
dc.abstract.enSalivary gland tumours (SGTs) are a heterogeneous group of benign tumours of various origins and pathologies, showing a number of DNA modifications. Previously, in malignant head and neck cancer (HNSCC), we found overexpression of ALKBH proteins, the homologs of Escherichia coli AlkB 2-oxoglutarate and Fe(II) dependent dioxygenase. Moreover, we proved the connection of some of these dioxygenases with cancer development. Here, we studied the expression of five of these ALKBH dioxygenases: 1, 3, 4, 5, and FTO in benign SGTs. Using Western blot analysis, we found overexpression of three proteins: ALKBH1, 4, and FTO in SGT as compared to the surrounding, unaffected tissue. ALKBH4 was overexpressed in 76% of patient samples, whereas ALKBH1 and FTO in 65% of the samples. These results differ from those obtained in HNSCC, where FTO overexpression has been observed in 90% of patient samples. We also investigated the relationships between ALKBHs’ expression levels in normal and SGT tissues and identified two correlated pairs: ALKBH1-ALKBH3 and ALKBH1-ALKBH5. Additionally, in tumour tissue ALKBHs: ALKBH1, ALKBH3, ALKBH4, and ALKBH5 levels were correlated with each other. Together, these findings show that the ALKBH proteins exhibit pro cancerogenic action in SGT, even though the levels ALKBHs are generally lower in benign SGT than in malignant HNSCC. We suggest that the overexpression of the ALKBHs, especially FTO, may be used as a cancer marker and for its grading.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorMarcinkowski, Michał
dc.contributor.authorGrzesiuk, Elżbieta
dc.contributor.authorKukwa, Wojciech
dc.contributor.authorPilžys, Tomaš
dc.contributor.authorGarbicz, Damian
dc.date.accessioned2024-01-26T07:30:47Z
dc.date.available2024-01-26T07:30:47Z
dc.date.copyright2022-12-02
dc.date.issued2022
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number4
dc.description.versionFINAL_PUBLISHED
dc.description.volume69
dc.identifier.doi10.18388/ABP.2020_6486
dc.identifier.issn0001-527X
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/119538
dc.identifier.weblinkhttps://ojs.ptbioch.edu.pl/index.php/abp/article/download/6486/5510
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofActa Biochimica Polonica
dc.relation.pages889–894
dc.rightsCC-BY-NC-SA
dc.sciencecloudnosend
dc.subject.ensalivary gland tumours,
dc.subject.enALKBH proteins,
dc.subject.enWestern blot
dc.titleSelected ALKBH dioxygenases are overexpressed in salivary gland tumours
dc.typeJournalArticle
dspace.entity.typePublication