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Cfap91-Dependent Stability of the RS2 and RS3 Base Proteins and Adjacent Inner Dynein Arms in Tetrahymena Cilia

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dc.abstract.enMotile cilia and eukaryotic flagella are specific cell protrusions that are conserved from protists to humans. They are supported by a skeleton composed of uniquely organized microtubules—nine peripheral doublets and two central singlets (9 × 2 + 2). Microtubules also serve as docking sites for periodically distributed multiprotein ciliary complexes. Radial spokes, the T-shaped ciliary complexes, repeat along the outer doublets as triplets and transduce the regulatory signals from the cilium center to the outer doublet-docked dynein arms. Using the genetic, proteomic, and microscopic approaches, we have shown that lack of Tetrahymena Cfap91 protein affects stable docking/positioning of the radial spoke RS3 and the base of RS2, and adjacent inner dynein arms, possibly due to the ability of Cfap91 to interact with a molecular ruler protein, Ccdc39. The localization studies confirmed that the level of RS3-specific proteins, Cfap61 and Cfap251, as well as RS2-associated Cfap206, are significantly diminished in Tetrahymena CFAP91-KO cells. Cilia of Tetrahymena cells with knocked-out CFAP91 beat in an uncoordinated manner and their beating frequency is dramatically reduced. Consequently, CFAP91-KO cells swam about a hundred times slower than wild-type cells. We concluded that Tetrahymena Cfap91 localizes at the base of radial spokes RS2 and RS3 and likely plays a role in the radial spoke(s) positioning and stability.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKonopka, Anna
dc.contributor.authorBulska, Ewa
dc.contributor.authorWŁOGA, DOROTA
dc.contributor.authorUrbańska, Paulina
dc.contributor.authorOsinka, Anna
dc.contributor.authorJOACHIMIAK, EWA
dc.contributor.authorBicka, Marta
dc.date.accessioned2024-01-24T19:05:22Z
dc.date.available2024-01-24T19:05:22Z
dc.date.copyright2022-12-14
dc.date.issued2022
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number24
dc.description.versionFINAL_PUBLISHED
dc.description.volume11
dc.identifier.doi10.3390/CELLS11244048
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/102827
dc.identifier.weblinkhttps://www.mdpi.com/2073-4409/11/24/4048/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofCells
dc.relation.pages4048
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enaxoneme
dc.subject.enCFAP91
dc.subject.encilia
dc.subject.enradial spoke
dc.subject.enTetrahymena
dc.titleCfap91-Dependent Stability of the RS2 and RS3 Base Proteins and Adjacent Inner Dynein Arms in Tetrahymena Cilia
dc.typeJournalArticle
dspace.entity.typePublication