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Dostęp zamkniętyDelivery of chicken egg ovalbumin to dendritic cells by listeriolysin O-secreting vegetative Bacillus subtilis
| cris.lastimport.scopus | 2024-02-12T20:11:51Z |
| dc.abstract.en | Listeriolysin O (LLO), one of the most immunogenic proteins of Listeria monocytogenes and its main virulence factor, mediates bacterial escape from the phagosome of infected cell. Thus, its expression in non-pathogenic bacterial host may enable effective delivery of heterologous antigens to the host cell cytosol and lead to their processing predominantly through the cytosolic MHC class I presentation pathway. The aim of this project was to characterize a delivery of a model antigen, chicken egg ovalbumin (OVA) to the cytosol of dendritic cells by recombinant Bacillus subtilis vegetative cells expressing LLO. Our work indicates that LLO produced by non-sporulating, vegetative bacteria was able to support OVA epitope presentation by MHC I molecules on the surface of antigen presenting cells and consequently influenced OVA-specific cytotoxic T cells activation. Additionally, it was proven that genetic context of epitope sequence is of great importance, as only native, full-sequence OVA fused to N-terminal fragment of LLO was sufficient for effective epitope delivery and activation of CD8+ lymphocytes. These results demonstrate necessity for further verification of a fusion antigen potency of enhancing the MHC I presentation and they prove that LLO producing B. subtilis may represent a novel and attractive candidate for a vaccine vector. |
| dc.affiliation | Uniwersytet Warszawski |
| dc.contributor.author | Jagielski, Tomasz |
| dc.contributor.author | Roeske, Katarzyna |
| dc.contributor.author | Stachowiak, Radosław |
| dc.contributor.author | Kamiński, Michał |
| dc.contributor.author | Bielecki, Jacek |
| dc.date.accessioned | 2024-01-24T21:27:51Z |
| dc.date.available | 2024-01-24T21:27:51Z |
| dc.date.issued | 2018 |
| dc.description.finance | Nie dotyczy |
| dc.identifier.doi | 10.4014/JMB.1706.06057 |
| dc.identifier.issn | 1017-7825 |
| dc.identifier.uri | https://repozytorium.uw.edu.pl//handle/item/104503 |
| dc.language | eng |
| dc.pbn.affiliation | biological sciences |
| dc.relation.ispartof | Journal of Microbiology and Biotechnology |
| dc.rights | ClosedAccess |
| dc.sciencecloud | nosend |
| dc.title | Delivery of chicken egg ovalbumin to dendritic cells by listeriolysin O-secreting vegetative Bacillus subtilis |
| dc.type | JournalArticle |
| dspace.entity.type | Publication |