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SERS studies of adsorption on gold surfaces of mononucleotides with attached hexanethiol moiety: Comparison with selected single-stranded thiolated DNA fragments

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dc.abstract.enThe attachment of DNA strands to gold surfaces is performed in many devices, such as various DNA sensors. One of the standard methods used to immobilize DNA on gold surfaces involves two steps: the attachment of a thiol linker group (usually in the form of alkanethiol moiety) to the DNA strand, and the chemical reaction between the thiol-terminated DNA and the gold surface. Since thiols react chemically with the surface of gold substrates, forming very stable Au–S bonds, it is often assumed that the chemisorption on the gold surface of nucleotides with an attached thiol linker group leads to the formation of an order layer with the linking moieties relatively densely packed on the gold surface. In this contribution we show that chemisorption of thiolated mononucleotides does not occur according to this model. For example, the thiolated mononucleotide containing adenine strongly interacts with the gold surface via the adenine moiety. Moreover, bonding of the mononucleotide containing adenine to the gold surface is relatively similar to the bonding of adenine, and the main difference is that the adenine interacts with the gold surface mainly through the pyrimidine ring, while for adenine mononucleotide interaction via the imidazole ring also significantly contributes to the total bonding. A similar effect was observed for the mononucleotide containing cytosine, and the main difference between the interaction with the gold surface of cytosine and cytosine mononucleotide is that mononucleotide containing cytosine interacts with the gold surface to a significantly larger extend via the carboxylic group of the base. We also show that the structure of the layer formed on the gold surface by the thiolated mononucleotides may be significantly different than the structure of the layer formed by thiolated single-stranded DNA containing even as few as two bases.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKudelski, Andrzej
dc.contributor.authorSzaniawska, Aleksandra
dc.contributor.authorPyrak, Edyta
dc.date.accessioned2024-01-26T07:34:42Z
dc.date.available2024-01-26T07:34:42Z
dc.date.copyright2019-10-30
dc.date.issued2019
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number21
dc.description.versionFINAL_PUBLISHED
dc.description.volume24
dc.identifier.doi10.3390/MOLECULES24213921
dc.identifier.issn1420-3049
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/119672
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofMolecules
dc.relation.pagesart. no. 3921 1-15
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enRaman spectroscopy
dc.subject.ensurface-enhanced Raman spectroscopy
dc.subject.enSERS
dc.subject.enalkanethiols
dc.subject.enmononucleotides
dc.subject.enDNA
dc.titleSERS studies of adsorption on gold surfaces of mononucleotides with attached hexanethiol moiety: Comparison with selected single-stranded thiolated DNA fragments
dc.typeJournalArticle
dspace.entity.typePublication