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Decapping Scavenger Enzyme Activity toward N2-Substituted 5′ End mRNA Cap Analogues

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dc.abstract.enmRNA degradation is a key mechanism of gene expression regulation. In the 3′ → 5′ decay pathway, mRNA is degraded by the exosome complex and the resulting cap dinucleotide or short-capped oligonucleotide is hydrolyzed mainly by a decapping scavenger enzyme (DcpS) - a member of the histidine triad family. The decapping mechanism is similar for DcpS from different species; however, their respective substrate specificities differ. In this paper, we describe experiments exploring DcpS activity from human (hDcps), Caenorhabditis elegans (CeDcpS), and Ascaris suum (AsDcpS) toward dinucleotide cap analogues modified at the N2 position of 7-methylguanosine. Various alkyl substituents were tested, and cap analogues with a longer than three-carbon chain were nonhydrolyzable by hDcpS and CeDcpS. Resistance of the modified cap analogues to hDcpS and CeDcpS may be associated with their weaker binding with enzymes.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorPietrow, Paulina
dc.contributor.authorFerenc-Mrozek, Aleksandra
dc.contributor.authorBojarska, Elzbieta
dc.contributor.authorPiecyk, Karolina
dc.contributor.authorJankowska-Anyszka, Marzena
dc.contributor.authorDarżynkiewicz, Edward
dc.date.accessioned2024-01-24T21:17:43Z
dc.date.available2024-01-24T21:17:43Z
dc.date.issued2019
dc.description.financeNie dotyczy
dc.description.number17
dc.description.volume4
dc.identifier.doi10.1021/ACSOMEGA.9B02715
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/104376
dc.identifier.weblinkhttp://pubs.acs.org/doi/pdf/10.1021/acsomega.9b02715
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofACS Omega
dc.relation.pages17576-17580
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.titleDecapping Scavenger Enzyme Activity toward N2-Substituted 5′ End mRNA Cap Analogues
dc.typeJournalArticle
dspace.entity.typePublication