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Hydrolytic activity of human Nudt16 enzyme on dinucleotide cap analogs and short capped oligonucleotides.

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cris.lastimport.scopus2024-02-12T20:08:30Z
dc.abstract.enHuman Nudt16 (hNudt16) is a member of the Nudix family of hydrolases, comprising enzymes catabolizing various substrates including canonical (d)NTPs, oxidized (d)NTPs, nonnucleoside polyphosphates, and capped mRNAs. Decapping activity of the Xenopus laevis (X29) Nudt16 homolog was observed in the nucleolus, with a high specificity toward U8 snoRNA. Subsequent studies have reported cytoplasmic localization of mammalian Nudt16 with cap hydrolysis activity initiating RNA turnover, similar to Dcp2. The present study focuses on hNudt16 and its hydrolytic activity toward dinucleotide cap analogs and short capped oligonucleotides. We performed a screening assay for potential dinucleotide and oligonucleotide substrates for hNudt16. Our data indicate that dinucleotide cap analogs and capped oligonucleotides containing guanine base in the first transcribed nucleotide are more susceptible to enzymatic digestion by hNudt16 than their counterparts containing adenine. Furthermore, unmethylated dinucleotides (GpppG and ApppG) and respective oligonucleotides (GpppG-16nt and GpppA-16nt) were hydrolyzed by hNudt16 with greater efficiency than were m7GpppG and m7GpppG-16nt. In conclusion, we found that hNudt16 hydrolysis of dinucleotide cap analogs and short capped oligonucleotides displayed a broader spectrum specificity than is currently known.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorTyras, Michał
dc.contributor.authorStępiński, Janusz
dc.contributor.authorGrzela, Renata
dc.contributor.authorBojarska, Elżbieta
dc.contributor.authorJankowska-Anyszka, Marzena
dc.contributor.authorŁukaszewicz, Maciej
dc.contributor.authorDarżynkiewicz, Edward
dc.contributor.authorNasiłowska, Karolina
dc.date.accessioned2024-01-25T03:31:46Z
dc.date.available2024-01-25T03:31:46Z
dc.date.issued2018
dc.description.financeNie dotyczy
dc.description.number5
dc.description.volume24
dc.identifier.doi10.1261/RNA.065698.118
dc.identifier.issn1355-8382
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/108800
dc.languageeng
dc.relation.ispartofRNA
dc.relation.pages633-642
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enCap analogs
dc.subject.enCap hydrolysis
dc.subject.enCapped oligonucleotides
dc.subject.enDecapping enzymes
dc.subject.enNudix hydrolases
dc.titleHydrolytic activity of human Nudt16 enzyme on dinucleotide cap analogs and short capped oligonucleotides.
dc.typeJournalArticle
dspace.entity.typePublication