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Molecular characterization of the pSinB plasmid of the arsenite oxidizing, metallotolerant Sinorhizobium sp. M14 - insight into the heavy metal resistome of sinorhizobial extrachromosomal replicons

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dc.abstract.enSinorhizobium sp. M14 is an As(III)-oxidizing, psychrotolerant strain, capable of growth in the presence of extremely high concentrations of arsenic and many other heavy metals. Metallotolerant abilities of the M14 strain depend upon the presence of two extrachromosomal replicons, pSinA (∼ 109 kb) and pSinB (∼ 300 kb). The latter was subjected to complex analysis. The performed analysis demonstrated that the plasmid pSinB is a narrow-host-range repABC-type replicon, which is fully stabilized by the phd-vapC-like toxin-antitoxin stabilizing system. In silico analysis showed that among the phenotypic gene clusters of the plasmid pSinB, eight modules are potentially involved in heavy metals resistance (HMR). These modules carry genes encoding efflux pumps, permeases, transporters and copper oxidases, which provide resistance to arsenic, cadmium, cobalt, copper, iron, mercury, nickel, silver and zinc. The functional analysis revealed that the HMR modules are active and have an effect on the minimal inhibitory concentrations (MICs) values observed for the heterological host cells. The phenotype was manifested by an increase or decrease of the MICs of heavy metals and it was strain specific. The analysis of distribution of the heavy metal resistance genes, i.e. resistome, in Sinorhizobium spp. plasmids, revealed that the HMR modules are common in these replicons.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorDziewit, Łukasz
dc.contributor.authorRadlińska, Monika
dc.contributor.authorDrewniak, Łukasz
dc.contributor.authorMielnicki, Sebastian
dc.contributor.authorRomaniuk, Krzysztof
dc.contributor.authorDecewicz, Przemysław
dc.date.accessioned2024-01-25T12:53:08Z
dc.date.available2024-01-25T12:53:08Z
dc.date.copyright2018-12-11
dc.date.issued2017
dc.date.openaccess12
dc.description.accesstimeAFTER_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number1
dc.description.versionFINAL_PUBLISHED
dc.description.volume93
dc.identifier.doi10.1093/FEMSEC/FIW215
dc.identifier.issn0168-6496
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/112854
dc.identifier.weblinkhttps://academic.oup.com/femsec/article/93/1/fiw215/2666415
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofFEMS Microbiology Ecology
dc.relation.pagesfiw215
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.engenomics
dc.subject.enplasmid pSinB Sinorhizobium sp. M14 heavy metal resistome
dc.subject.enrepABC system
dc.subject.entoxin-antitoxin system
dc.titleMolecular characterization of the pSinB plasmid of the arsenite oxidizing, metallotolerant Sinorhizobium sp. M14 - insight into the heavy metal resistome of sinorhizobial extrachromosomal replicons
dc.typeJournalArticle
dspace.entity.typePublication