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The study of complexation between dicationic surfactants and the DNA duplex using structural and spectroscopic methods

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cris.lastimport.scopus2024-02-12T19:42:56Z
dc.abstract.enDicationic (also known as gemini or dimeric) bis-alkylimidazolium surfactants belong to a group of non-viral transfection systems proposed for the successful introduction of different types of nucleic acids (i.e., siRNA, DNA oligomers, and plasmid DNA) into living cells. Our studies reveal the formation of complexes composed of dicationic (gemini) surfactants, 3,3′-[α,ω-(dioxaalkane)]bis(1-dodecylimidazolium)chlorides, and 21 base pair deoxyribonucleic acid duplexes (dsDNA). The studied dsDNA and its complex formation process was analysed by small-angle X-ray scattering (SAXS), molecular modelling (dsDNA), agarose gel electrophoresis (AGE) and circular dichroism spectroscopy (CD). We observed the formation of stable complexes for charge ratio values of p/n > 2. Moreover, we noted conformational changes similar to those observed during the transition of B-DNA to C-DNA, X-DNA, and Z-DNA in several spatial structures (i.e., micellar, hexagonal and cubic) formed in mixtures. The surfactants used in this study were investigated for the influence of dioxaalkane spacer length and the presence of an imidazolium moiety on the complexation process. The complexes formed were stable, and the complexation process was reproducible and efficient. Toxicity tests done on HeLa cells allowed for the determination of non-toxic concentrations of studied surfactants. Transfection tests have confirmed that the studied surfactant systems are effective DNA carriers. © The Royal Society of Chemistry 2017.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorWilkowska, Michalina
dc.contributor.authorKozak, Maciej
dc.contributor.authorAndrzejewska, Weronika
dc.contributor.authorChrabąszczewska, Magdalena
dc.date.accessioned2024-01-26T10:48:46Z
dc.date.available2024-01-26T10:48:46Z
dc.date.issued2017
dc.description.financeNie dotyczy
dc.identifier.doi10.1039/C6RA24978G
dc.identifier.issn2046-2069
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/123199
dc.pbn.affiliationbiological sciences
dc.relation.ispartofRSC Advances
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.titleThe study of complexation between dicationic surfactants and the DNA duplex using structural and spectroscopic methods
dc.typeJournalArticle
dspace.entity.typePublication