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Ancient Bacterial Class Alphaproteobacteria Cytochrome P450 Monooxygenases Can Be Found in Other Bacterial Species

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dc.abstract.enCytochrome P450 monooxygenases (CYPs/P450s), heme-thiolate proteins, are well-known players in the generation of chemicals valuable to humans and as a drug target against pathogens. Understanding the evolution of P450s in a bacterial population is gaining momentum. In this study, we report comprehensive analysis of P450s in the ancient group of the bacterial class Alphaproteobacteria. Genome data mining and annotation of P450s in 599 alphaproteobacterial species belonging to 164 genera revealed the presence of P450s in only 241 species belonging to 82 genera that are grouped into 143 P450 families and 214 P450 subfamilies, including 77 new P450 families. Alphaproteobacterial species have the highest average number of P450s compared to Firmicutes species and cyanobacterial species. The lowest percentage of alphaproteobacterial species P450s (2.4%) was found to be part of secondary metabolite biosynthetic gene clusters (BGCs), compared other bacterial species, indicating that during evolution large numbers of P450s became part of BGCs in other bacterial species. Our study identified that some of the P450 families found in alphaproteobacterial species were passed to other bacterial species. This is the first study to report on the identification of CYP125 P450, cholesterol and cholest-4-en-3-one hydroxylase in alphaproteobacterial species (Phenylobacterium zucineum) and to predict cholesterol side-chain oxidation capability (based on homolog proteins) by P. zucineum.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorGront, Dominik
dc.contributor.authorNzuza, Nomfundo
dc.contributor.authorKryś, Justyna Dorota
dc.contributor.authorNelson, David R.
dc.contributor.authorSyed, Khajamohiddin
dc.contributor.authorChen, Wanping
dc.contributor.authorSyed, Puleng Rosinah
dc.contributor.authorPadayachee, Tiara
dc.date.accessioned2024-01-24T16:34:25Z
dc.date.available2024-01-24T16:34:25Z
dc.date.copyright2021-05-24
dc.date.issued2021
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number11
dc.description.versionFINAL_PUBLISHED
dc.description.volume22
dc.identifier.doi10.3390/IJMS22115542
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/100664
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/22/11/5542/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages5542
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.encytochrome P450 monooxygenase
dc.subject.engenome data mining
dc.subject.enannotation
dc.subject.enAlphaproteobacteria
dc.subject.enbiosynthetic gene clusters
dc.subject.enCYP125
dc.subject.encholesterol oxidation
dc.titleAncient Bacterial Class Alphaproteobacteria Cytochrome P450 Monooxygenases Can Be Found in Other Bacterial Species
dc.typeJournalArticle
dspace.entity.typePublication