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Antibody–Antigen Interaction Dynamics Revealed by Analysis of Single-Molecule Equilibrium Fluctuations on Individual Plasmonic Nanoparticle Biosensors

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dc.abstract.enAntibody–antigen interactions are complex events central to immune response, in vivo and in vitro diagnostics, and development of therapeutic substances. We developed an ultrastable single-molecule localized surface plasmon resonance (LSPR) sensing platform optimized for studying antibody–antigen interaction kinetics over very long time scales. The setup allowed us to perform equilibrium fluctuations analysis of the PEG/anti-PEG interaction. By time and frequency domain analysis, we demonstrate that reversible adsorption of monovalently bound anti-PEG antibodies is the dominant factor affecting the LSPR fluctuations. The results suggest that equilibrium fluctuation analysis can be an alternative to established methods for determination of interaction rates. In particular, the methodology is suited to analyze molecular systems whose properties change during the initial interaction phases, for example, due to mass transport limitations or, as demonstrated here, because the effective association rate constant varies with surface concentration of adsorbed molecules.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorAntosiewicz, Tomasz
dc.contributor.authorAćimovic, Srdjan S.
dc.contributor.authorŠípová-Jungová, Hana
dc.contributor.authorEmilsson, Gustav
dc.contributor.authorShao, Lei
dc.contributor.authorDahlin, Andreas B.
dc.contributor.authorKäll, Mikael
dc.date.accessioned2024-01-24T16:40:09Z
dc.date.available2024-01-24T16:40:09Z
dc.date.issued2018
dc.description.financeNie dotyczy
dc.description.number10
dc.description.volume12
dc.identifier.doi10.1021/ACSNANO.8B04016
dc.identifier.issn1936-0851
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/100775
dc.identifier.weblinkhttps://pubs.acs.org/doi/10.1021/acsnano.8b04016#
dc.languageeng
dc.pbn.affiliationphysical sciences
dc.relation.ispartofACS Nano
dc.relation.pages9958-9965
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enanti-PEG
dc.subject.enbiosensors
dc.subject.enequilibrium fluctuations
dc.subject.ennanoparticles
dc.subject.enPEG
dc.subject.enreaction rate constants
dc.titleAntibody–Antigen Interaction Dynamics Revealed by Analysis of Single-Molecule Equilibrium Fluctuations on Individual Plasmonic Nanoparticle Biosensors
dc.typeJournalArticle
dspace.entity.typePublication