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Physicochemical and Biological Characterization of Novel Membrane-Active Cationic Lipopeptides with Antimicrobial Properties

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dc.abstract.enWe have designed and synthesized new short lipopeptides composed of tetrapeptide conjugated to fatty acids with different chain lengths. The amino acid sequence of the peptide moiety included D-phenylalanine, two residues of L-2,4diaminobutyric acid and L-leucine. To explore the possible mechanism of lipopeptide action, we have provided a physicochemical characterization of their interactions with artificial lipid membranes. For this purpose, we have used monolayers and bilayers composed of lipids representative of Gram-negative and Gram-positive bacterial membranes. Using surface pressure measurements and atomic force microscopy, we were able to monitor the changes occurring within the films upon exposure to lipopeptides. Our experiments revealed that all lipopeptides can penetrate the lipid membranes and affect their molecular ordering. The latter results in membrane thinning and fluidization. However, the effect is stronger in the lipid films mimicking Gram-positive bacterial membranes. The results of the physicochemical characterization were compared with the biological activity of lipopeptides. The effect of lipopeptides on bacterial growth was tested on several strains of bacteria. It was revealed that lipopeptides show stronger antimicrobial activity against Gram-positive bacteria. At the same time, all tested compounds display relatively low hemolytic activity.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorJuhaniewicz-Dębińska, Joanna
dc.contributor.authorTymecka, Dagmara
dc.contributor.authorSęk, Sławomir
dc.contributor.authorLasek, Robert
dc.contributor.authorBurdach, Kinga
dc.contributor.authorBartosik, Dariusz
dc.date.accessioned2024-01-25T16:37:29Z
dc.date.available2024-01-25T16:37:29Z
dc.date.copyright2020-10-21
dc.date.issued2020
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number43
dc.description.versionFINAL_PUBLISHED
dc.description.volume36
dc.identifier.doi10.1021/ACS.LANGMUIR.0C02135
dc.identifier.issn0743-7463
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/115886
dc.identifier.weblinkhttp://pubs.acs.org/doi/pdf/10.1021/acs.langmuir.0c02135
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofLangmuir
dc.relation.pages12900-12910
dc.rightsCC-BY
dc.sciencecloudnosend
dc.titlePhysicochemical and Biological Characterization of Novel Membrane-Active Cationic Lipopeptides with Antimicrobial Properties
dc.typeJournalArticle
dspace.entity.typePublication