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Ovarian carcinoma immunoreactive antigen–like protein 2 (OCIAD2) is a novel complex III–specific assembly factor in mitochondria

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dc.abstract.enAssembly of the dimeric complex III (CIII2) in the mitochondrial inner membrane is an intricate process in which several accessory proteins are involved as assembly factors. Despite numerous studies, this process has yet to be fully understood. Here we report the identification of human OCIAD2 (ovarian carcinoma immunoreactive antigen–like protein 2) as an assembly factor for CIII2. OCIAD2 was found to be deregulated in several carcinomas and also in some neurogenerative disorders; however, its nonpathological role had not been elucidated. We have shown that OCIAD2 localizes to mitochondria and interacts with electron transport chain (ETC) proteins. Complete loss of OCIAD2 using gene editing in HEK293 cells resulted in abnormal mitochondrial morphology, a substantial decrease of both CIII2 and supercomplex III2+IV, and a reduction in CIII enzymatic activity. Identification of OCIAD2 as a protein required for assembly of functional CIII2 provides a new insight into the biogenesis and architecture of the ETC. Elucidating the mechanism of OCIAD2 action is important both for the understanding of cellular metabolism and for an understanding of its role in malignant transformation.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorGóral, Tomasz
dc.contributor.authorChojnacka, Katarzyna
dc.contributor.authorChacińska, Agnieszka
dc.contributor.authorSerwa, Remigiusz
dc.contributor.authorRehling, Peter
dc.contributor.authorSzczepanowska, Karolina
dc.contributor.authorBanach, Tomasz
dc.contributor.authorGosk, Aleksandra
dc.contributor.authorCallegari, Sylvie
dc.contributor.authorMohanraj, Karthik
dc.contributor.authorMussulini, Ben Hur Marins
dc.contributor.authorElancheliyan, Praveenraj
dc.date.accessioned2024-01-25T16:10:04Z
dc.date.available2024-01-25T16:10:04Z
dc.date.copyright2022-05-17
dc.date.issued2022
dc.date.openaccess2
dc.description.accesstimeAFTER_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number4
dc.description.versionFINAL_PUBLISHED
dc.description.volume33
dc.identifier.doi10.1091/MBC.E21-03-0143
dc.identifier.issn1059-1524
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/115133
dc.identifier.weblinkhttp://dx.doi.org/10.1091/mbc.e21-03-0143
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofMolecular Biology of the Cell
dc.rightsCC-BY-NC-SA
dc.sciencecloudnosend
dc.titleOvarian carcinoma immunoreactive antigen–like protein 2 (OCIAD2) is a novel complex III–specific assembly factor in mitochondria
dc.typeJournalArticle
dspace.entity.typePublication