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Vitamin B12 transports modified RNA into E. coli and S. Typhimurium cells

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cris.lastimport.scopus2024-02-12T19:40:18Z
dc.abstract.enSpecifically designed, antisense oligonucleotides are promising candidates for antibacterial drugs. They suppress the correct expression of bacterial genes by complementary binding to essential sequences of bacterial DNA or RNA. The main obstacle in fully utilizing their potential as therapeutic agents comes from the fact that bacteria do not uptake oligonucleotides from their environment. Herein, we report that vitamin B12 can transport oligonucleotides into Escherichia coli and Salmonella typhimurium cells. 5′-Aminocobalamin with an alkyne linker and azide-modified oligonucleotides enabled the synthesis of vitamin B12–2′OMeRNA conjugates using an efficient “click” methodology. Inhibition of protein expression in E. coli and S. Typhimurium cells indicates an unprecedented transport of 2′OMeRNA oligomers into bacterial cells via the vitamin B12 delivery pathway.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorTrylska, Joanna
dc.contributor.authorJackowska, Agnieszka
dc.contributor.authorKolanowska, Monika
dc.contributor.authorRównicki, Marcin
dc.contributor.authorGRYKO, Dorota
dc.contributor.authorGiedyk, Maciej
dc.date.accessioned2024-01-26T11:49:40Z
dc.date.available2024-01-26T11:49:40Z
dc.date.issued2019
dc.description.financeNie dotyczy
dc.description.volume55
dc.identifier.doi10.1039/C8CC05064C
dc.identifier.issn1359-7345
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/124686
dc.identifier.weblinkhttp://dx.doi.org/10.1039/C8CC05064C
dc.languageeng
dc.pbn.affiliationmedical sciences
dc.relation.ispartofChemical Communications
dc.relation.pages763-766
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.titleVitamin B12 transports modified RNA into E. coli and S. Typhimurium cells
dc.typeJournalArticle
dspace.entity.typePublication