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DNA Repair Protein APE1 Degrades Dysfunctional Abasic mRNA in Mitochondria Affecting Oxidative Phosphorylation.

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cris.lastimport.scopus2024-02-12T20:54:52Z
dc.abstract.enAPE1 is a multifunctional protein which plays a central role in the maintenance of nuclear and mitochondrial genomes repairing DNA lesions caused by oxidative and alkylating agents. In addition, it works as a redox signaling protein regulating gene expression by interacting with many transcriptional factors. Apart from these canonical activities, recent studies have shown that APE1 is also enzymatically active on RNA molecules. The present study unveils for the first time a new role of the mitochondrial form of APE1 protein in the metabolism of RNA in mitochondria. Our data demonstrate that APE1 is associated with mitochondrial messenger RNA and exerts endoribonuclease activity on abasic sites. Loss of APE1 results in the accumulation of damaged mitochondrial mRNA species, determining impairment in protein translation and reduced expression of mitochondrial-encoded proteins, finally leading to less efficient mitochondrial respiration. Altogether, our data demonstrate that APE1 plays an active role in the degradation of the mitochondrial mRNA and has a profound impact on mitochondrial well-being.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorWarscheid, Bettina
dc.contributor.authorOeljeklaus, Silke
dc.contributor.authorBarchiesi, Arianna
dc.contributor.authorBazzani, Veronica
dc.contributor.authorVascotto, Carlo
dc.contributor.authorBorowski, Łukasz
dc.contributor.authorSzczęsny, Roman
dc.contributor.authorJabczyńska, Agata
dc.contributor.authorChacińska, Agnieszka
dc.date.accessioned2024-01-24T22:11:28Z
dc.date.available2024-01-24T22:11:28Z
dc.date.copyright2021-07-02
dc.date.issued2021
dc.description.accesstimeBEFORE_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number18
dc.description.versionFINAL_PUBLISHED
dc.description.volume433
dc.identifier.doi10.1016/J.JMB.2021.167125
dc.identifier.issn0022-2836
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/105107
dc.identifier.weblinkhttps://doi.org/10.1016/j.jmb.2021.167125
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofJournal of Molecular Biology
dc.relation.pages167125 (1-21)
dc.rightsCC-BY-NC-ND
dc.sciencecloudnosend
dc.subject.enapurinic/apyrimidinic endonuclease 1
dc.subject.enmitochondria
dc.subject.enoxidative phosphorylation
dc.subject.enRNA processing
dc.titleDNA Repair Protein APE1 Degrades Dysfunctional Abasic mRNA in Mitochondria Affecting Oxidative Phosphorylation.
dc.typeJournalArticle
dspace.entity.typePublication