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Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans

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cris.lastimport.scopus2024-02-12T20:55:15Z
dc.abstract.enKae1 is a subunit of the highly evolutionarily conserved KEOPS/EKC complex, which is involved in universal (t6A37) tRNA modification. Several reports have discussed the participation of this complex in transcription regulation in yeast and human cells, including our previous observations of KaeA, an Aspergillus nidulans homologue of Kae1p. The aim of this project was to confirm the role of KaeA in transcription, employing high-throughput transcriptomic (RNA-Seq and ChIP-Seq) and proteomic (LC-MS) analysis. We confirmed that KaeA is a subunit of the KEOPS complex in A. nidulans. An analysis of kaeA19 and kaeA25 mutants showed that, although the (t6A37) tRNA modification is unaffected in both mutants, they reveal significantly altered transcriptomes compared to the wild type. The finding that KaeA is localized in chromatin and identifying its protein partners allows us to postulate an additional nuclear function for the protein. Our data shed light on the universal bi-functional role of this factor and proves that the activity of this protein is not limited to tRNA modification in cytoplasm, but also affects the transcriptional activity of a number of nuclear genes. Data are available via the NCBI’s GEO database under identifiers GSE206830 (RNA-Seq) and GSE206874 (ChIP-Seq), and via ProteomeXchange with identifier PXD034554 (proteomic).
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKoper, Michał
dc.contributor.authorDzikowska, Agnieszka
dc.contributor.authorWęgleński, Piotr
dc.contributor.authorBogdanowicz, Albert
dc.contributor.authorGawlik, Joanna
dc.date.accessioned2024-01-25T14:01:34Z
dc.date.available2024-01-25T14:01:34Z
dc.date.copyright2022-09-22
dc.date.issued2022
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number19
dc.description.versionFINAL_PUBLISHED
dc.description.volume23
dc.identifier.doi10.3390/IJMS231911138
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/114051
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/23/19/11138/pdf
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages11138 (1-20)
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enKEOPS/EKC complex
dc.subject.entranscription regulation
dc.subject.enRcoATup1 co-repressor
dc.titleNuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans
dc.typeJournalArticle
dspace.entity.typePublication