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Genoarchitecture of the Early Postmitotic Pretectum and the Role of Wnt Signaling in Shaping Pretectal Neurochemical Anatomy in Zebrafish

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cris.lastimport.scopus2024-02-12T19:35:07Z
dc.abstract.enThe pretectum has a distinct nuclear arrangement and complex neurochemical anatomy. While previous genoarchitectural studies have described rostrocaudal and dorsoventral progenitor domains and subdomains in different species, the relationship between these early partitions and its later derivatives in the mature anatomy is less understood. The signals and transcription factors that control the establishment of pretectal anatomy are practically unknown. We investigated the possibility that some aspects of the development of pretectal divisions are controlled by Wnt signaling, focusing on the transitional stage between neurogenesis and histogenesis in zebrafish. Using several molecular markers and following the prosomeric model, we identified derivatives from each rostrocaudal pretectal progenitor domain and described the localization of gad1b-positive GABAergic and vglut2.2-positive glutamatergic cell clusters. We also attempted to relate these clusters to pretectal nuclei in the mature brain. Then, we examined the influence of Wnt signaling on the size of neurochemically distinctive pretectal areas, using a chemical inhibitor of the Wnt pathway and the CRISPR/Cas9 approach to knock out genes that encode the Wnt pathway mediators, Lef1 and Tcf7l2. The downregulation of the Wnt pathway led to a decrease in two GABAergic clusters and an expansion of a glutamatergic subregion in the maturing pretectum. This revealed an instructive role of the Wnt signal in the development of the pretectum during neurogenesis. The molecular anatomy presented here improves our understanding of pretectal development during early postmitotic stages and support the hypothesis that Wnt signaling is involved in shaping the neurochemical organization of the pretectum. Keywords: zebrafish, brain, prosomeric model, pretectum, Wnt signaling, LEF1, TCF7L2
dc.affiliationUniwersytet Warszawski
dc.contributor.authorWiśniewska, Marta
dc.contributor.authorChakraborty, Chaitali
dc.contributor.authorGabriel, Michael
dc.contributor.authorSzewczyk, Łukasz
dc.contributor.authorBaggio, Suelen
dc.contributor.authorFerran, José L.
dc.contributor.authorJankowska, Marta
dc.contributor.authorLipiec, Marcin A.
dc.contributor.authorBrożko, Nikola
dc.date.accessioned2024-01-25T02:05:26Z
dc.date.available2024-01-25T02:05:26Z
dc.date.issued2022
dc.description.financeŚrodki finansowe, o których mowa w art. 365 pkt. 2 ustawy
dc.description.volume16
dc.identifier.doi10.3389/FNANA.2022.838567
dc.identifier.issn1662-5129
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/107834
dc.identifier.weblinkhttps://www.frontiersin.org/articles/10.3389/fnana.2022.838567/full
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofFrontiers in Neuroanatomy
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.titleGenoarchitecture of the Early Postmitotic Pretectum and the Role of Wnt Signaling in Shaping Pretectal Neurochemical Anatomy in Zebrafish
dc.typeJournalArticle
dspace.entity.typePublication