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Allogenic adipose-derived stem cells in diabetic foot ulcer treatment: clinical effectiveness, safety, survival in the wound site, and proteomic impact.

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cris.lastimport.scopus2024-02-12T19:35:27Z
dc.abstract.enAlthough encouraging results of adipose-derived stem cell (ADSC) use in wound healing are available, the mechanism of action has been studied mainly in vitro and in animals. This work aimed to examine the safety and efficacy of allogenic ADSCs in human diabetic foot ulcer treatment, in combination with the analyses of the wound. Equal groups of 23 participants each received fibrin gel with ADSCs or fibrin gel alone. The clinical effects were assessed at four time points: days 7, 14, 21 and 49. Material collected during debridement from a subset of each group was analyzed for the presence of ADSC donor DNA and proteomic changes. The reduction in wound size was greater at all subsequent visits, significantly on day 21 and 49, and the time to 50% reduction in the wound size was significantly shorter in patients who received ADSCs. Complete healing was achieved at the end of the study in seven patients treated with ADSCs vs. one treated without ADSCs. One week after ADSC application, 34 proteins significantly differentiated the material from both groups, seven of which, i.e., GAPDH, CAT, ACTN1, KRT1, KRT9, SCL4A1, and TPI, positively correlated with the healing rate. We detected ADSC donor DNA up to 21 days after administration. We confirmed ADSC-related improvement in wound healing that correlated with the molecular background, which provides insights into the role of ADSCs in wound healing—a step toward the development of cell-based therapies.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorZieliński, Jakub
dc.contributor.authorLewandowska-Szumiel, Malgorzata
dc.contributor.authorCzupryniak, Leszek
dc.contributor.authorPaleska, Barbara
dc.contributor.authorNoszczyk, Maria
dc.contributor.authorGrzela, Tomasz
dc.contributor.authorSieńko, Damian
dc.contributor.authorMieczkowski, Mateusz
dc.contributor.authorKonarzewska, Magdalena
dc.contributor.authorGasperowicz, Piotr
dc.contributor.authorPłoski, Rafał
dc.contributor.authorRudziński, Stefan
dc.contributor.authorCysewski, Dominik
dc.contributor.authorSzabłowska-Gadomska, Ilona
dc.contributor.authorMrozikiewicz-Rakowska, Beata
dc.date.accessioned2024-01-24T15:41:18Z
dc.date.available2024-01-24T15:41:18Z
dc.date.copyright2023-01-12
dc.date.issued2023
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number(2)
dc.description.versionFINAL_PUBLISHED
dc.description.volume24
dc.identifier.doi10.3390/IJMS24021472
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/100464
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/24/2/1472/pdf
dc.languageeng
dc.pbn.affiliationmedical sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages1-16
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enCell therapy
dc.subject.enDiabetic foot ulcer
dc.subject.enAdipose-derived stem cells
dc.subject.enProteomics
dc.subject.enClinical trials
dc.subject.enAllogenic cell therapy
dc.titleAllogenic adipose-derived stem cells in diabetic foot ulcer treatment: clinical effectiveness, safety, survival in the wound site, and proteomic impact.
dc.typeJournalArticle
dspace.entity.typePublication