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Plasmids of Psychrotolerant Polaromonas spp. Isolated From Arctic and Antarctic Glaciers - Diversity and Role in Adaptation to Polar Environments.

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cris.lastimport.scopus2024-02-12T19:51:39Z
dc.abstract.enCold-active bacteria of the genus Polaromonas (class Betaproteobacteria) are important components of glacial microbiomes. In this study, extrachromosomal replicons of 26 psychrotolerant Polaromonas strains, isolated from Arctic and Antarctic glaciers, were identified, sequenced, and characterized. The plasmidome of these strains consists of 13 replicons, ranging in size from 3,378 to 101,077 bp. In silico sequence analyses identified the conserved backbones of these plasmids, composed of genes required for plasmid replication, stable maintenance, and conjugal transfer. Host range analysis revealed that all of the identified plasmids are narrow-host-range replicons, only able to replicate in bacteria of closely related genera (Polaromonas and Variovorax) of the Comamonadaceae family. Special attention was paid to the identification of plasmid auxiliary genetic information, which may contribute to the adaptation of bacteria to environmental conditions occurring in glaciers. Detailed analysis revealed the presence of genes encoding proteins potentially involved in (i) protection against reactive oxygen species, ultraviolet radiation, and low temperatures; (ii) transport and metabolism of organic compounds; (iii) transport of metal ions; and (iv) resistance to heavy metals. Some of the plasmids also carry genes required for the molecular assembly of iron-sulfur [Fe-S] clusters. Functional analysis of the predicted heavy metal resistance determinants demonstrated that their activity varies, depending on the host strain. This study provides the first molecular insight into the mobile DNA of Polaromonas spp. inhabiting polar glaciers. It has generated valuable data on the structure and properties of a pool of plasmids and highlighted their role in the biology of psychrotolerant Polaromonas strains and their adaptation to the environmental conditions of Arctic and Antarctic glaciers.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorGawor, Jan
dc.contributor.authorDziewit, Łukasz
dc.contributor.authorGrzesiak, Jakub
dc.contributor.authorGromadka, Robert
dc.contributor.authorBartosik, Dariusz
dc.contributor.authorBudzik, Karol
dc.contributor.authorZdanowski, Marek
dc.contributor.authorDecewicz, Przemysław
dc.contributor.authorCiok, Anna
dc.date.accessioned2024-01-25T16:59:07Z
dc.date.available2024-01-25T16:59:07Z
dc.date.issued2018
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.versionFINAL_PUBLISHED
dc.description.volume9
dc.identifier.doi10.3389/FMICB.2018.01285
dc.identifier.issn1664-302X
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/116029
dc.identifier.weblinkhttps://www.frontiersin.org/articles/10.3389/fmicb.2018.01285/full
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofFrontiers in Microbiology
dc.relation.pages1285
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enPolaromonas, plasmid,
dc.subject.enAntarctica,
dc.subject.enbacterial adaptation,
dc.subject.enheavy metal resistance,
dc.subject.eniron–sulfur [Fe-S] cluster
dc.subject.enArctic,
dc.subject.englacier
dc.titlePlasmids of Psychrotolerant Polaromonas spp. Isolated From Arctic and Antarctic Glaciers - Diversity and Role in Adaptation to Polar Environments.
dc.typeJournalArticle
dspace.entity.typePublication