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CC-BY - Uznanie autorstwaThe Repeating, Modular Architecture of the HtrA Proteases
| dc.abstract.en | A conserved, 26-residue sequence [AA(X2)[A/G][G/L](X2)GDV[I/L](X2)[V/L]NGE(X1)V(X6)] and corresponding structure repeating module were identified within the HtrA protease family using a non-redundant set (N = 20) of publicly available structures. While the repeats themselves were far from sequence perfect, they had notable conservation to a statistically significant level. Three or more repetitions were identified within each protein despite being statistically expected to randomly occur only once per 1031 residues. This sequence repeat was associated with a six stranded antiparallel β-barrel module, two of which are present in the core of the structures of the PA clan of serine proteases, while a modified version of this module could be identified in the PDZ-like domains. Automated structural alignment methods had difficulties in superimposing these β-barrels, but the use of a target human HtrA2 structure showed that these modules had an average RMSD across the set of structures of less than 2 Å (mean and median). Our findings support Dayhoff’s hypothesis that complex proteins arose through duplication of simpler peptide motifs and domains |
| dc.affiliation | Uniwersytet Warszawski |
| dc.contributor.author | Górna, Maria |
| dc.contributor.author | Wieczorek, Rafał |
| dc.contributor.author | Macedo-Ribeiro, Sandra |
| dc.contributor.author | Merski, Matthew |
| dc.date.accessioned | 2024-01-26T10:37:16Z |
| dc.date.available | 2024-01-26T10:37:16Z |
| dc.date.copyright | 2022-06-07 |
| dc.date.issued | 2022 |
| dc.description.accesstime | BEFORE_PUBLICATION |
| dc.description.finance | Środki finansowe, o których mowa w art. 365 pkt. 2 ustawy |
| dc.description.number | 6 |
| dc.description.version | FINAL_PUBLISHED |
| dc.description.volume | 12 |
| dc.identifier.doi | 10.3390/BIOM12060793 |
| dc.identifier.uri | https://repozytorium.uw.edu.pl//handle/item/122977 |
| dc.identifier.weblink | https://www.mdpi.com/2218-273X/12/6/793/pdf |
| dc.language | eng |
| dc.pbn.affiliation | chemical sciences |
| dc.relation.ispartof | Biomolecules |
| dc.relation.pages | art.no. 793 |
| dc.rights | CC-BY |
| dc.sciencecloud | nosend |
| dc.subject.en | HtrA protease |
| dc.subject.en | PA clan |
| dc.subject.en | protein evolution |
| dc.subject.en | protein repeat |
| dc.subject.en | serine protease |
| dc.title | The Repeating, Modular Architecture of the HtrA Proteases |
| dc.type | JournalArticle |
| dspace.entity.type | Publication |