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Phenotypic plasticity of Escherichia coli upon exposure to physical stress induced by ZnO nanorods

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dc.abstract.enEvolution of bacteria to selective chemical pressure (e.g. antibiotics) is well studied in contrast to the influence of physical stressors. Here we show that instantaneous physical stress in a homogeneous environment (without concentration gradient) induces fast adaptation of Escherichia coli. We exposed E. coli to a large number of collisions of around 105 per bacterium per second with sharp ZnO nanorods. The pressure exerted on the bacterial cell wall was up to 10 GPa and induced phenotype changes. The bacteria's shape became more spherical, the density of their periplasm increased by around 15% and the average thickness of the cell wall by 30%. Such E. coli cells appeared almost as Gram-positive bacteria in the standard Gram staining. Additionally, we observed a combination of changes occurring at the genomic level (mutations identified in form of single nucleotide polymorphisms) and down-regulation of expression of 61 genes encoding proteins involved in β-oxidation of fatty acids, glycolysis, the citric acid cycle, as well as uptake of amino acids and enzyme cofactors. Thus, we show that bacteria undergo phenotypic changes upon instantaneous, acute physical stress without any obviously available time for gradual adaptation.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorDziewit, Łukasz
dc.contributor.authorBluyssen, Hans
dc.contributor.authorDerebecka, Natalia
dc.contributor.authorWYROBA, ELŻBIETA
dc.contributor.authorMatuła, Kinga
dc.contributor.authorPeplińska, Barbara
dc.contributor.authorHołyst, Robert
dc.contributor.authorGrzeszkowiak, Mikołaj
dc.contributor.authorSilesian, Adrian
dc.contributor.authorNogala, Wojciech
dc.contributor.authorŁoś, Joanna
dc.contributor.authorRichter, Łukasz
dc.contributor.authorŁoś, Marcin
dc.contributor.authorPaczesny, Jan
dc.contributor.authorWesoły, Joanna
dc.contributor.authorJurga, Stefan
dc.contributor.authorSUSKI, SZYMON
dc.contributor.authorBILSKI, HENRYK
dc.contributor.authorJanczuk-Richter, Marta
dc.contributor.authorDecewicz, Przemysław
dc.date.accessioned2024-01-25T16:36:09Z
dc.date.available2024-01-25T16:36:09Z
dc.date.issued2019
dc.description.financeNie dotyczy
dc.identifier.doi10.1038/S41598-019-44727-W
dc.identifier.issn2045-2322
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/115803
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofScientific Reports
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.titlePhenotypic plasticity of Escherichia coli upon exposure to physical stress induced by ZnO nanorods
dc.typeJournalArticle
dspace.entity.typePublication