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Brownian Motion Influence on AFM Exosomes’ Size Measurements

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dc.abstract.enExtracellular vesicles are evaluated by nanoparticle tracking analysis (NTA), providing information on their hydrodynamic diameters, and by atomic force microscopy (AFM) to calculate their geometric diameters. The aim of this study is to explore the influence of Brownian movements in a sample drop and preparation time on imaging-based measurements and to determine the relationship between the geometric and hydrodynamic sizes of the extracellular vesicles measured by the AFM and the NTA, respectively. Exosomes derived from the human prostate cancer cell line PC3 were evaluated by NTA and AFM, and those results were compared with Monte Carlo simulations. The mean size, evaluated by AFM shortly after application on the mica substrate, is less than its real value. It obtains the correct value faster for a thinner sample drop. Fitting the log-normal distribution to the geometric and hydrodynamic diameters leads to the conclusion that the latter could arise from the former by linear scaling by a factor that could be used to characterize the analyzed extracellular vesicles. The size of the vesicles attached to the mica substrate depends on time. The effect of Brownian motion and stretch of the lipid bilayer should be considered in the context of exosome AFM studies.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorGinter, Józef
dc.contributor.authorKamiński, Maciej
dc.contributor.authorBrzozowska, Beata
dc.contributor.authorPszczółkowska, Beata
dc.contributor.authorŻycieńska, Katarzyna
dc.contributor.authorSęk, Sławomir
dc.contributor.authorOlejarz, Wioletta
dc.contributor.authorLorenc, Tomasz
dc.date.accessioned2024-01-24T18:57:01Z
dc.date.available2024-01-24T18:57:01Z
dc.date.copyright2022-09-03
dc.date.issued2022
dc.description.accesstimeAT_PUBLICATION
dc.description.financeŚrodki finansowe, o których mowa w art. 365 pkt. 2 ustawy
dc.description.number17
dc.description.versionFINAL_PUBLISHED
dc.description.volume23
dc.identifier.doi10.3390/IJMS231710074
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/102568
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/23/17/10074/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages10074
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enextracellular vesicles
dc.subject.enexosomes
dc.subject.enprostate cancer
dc.subject.enatomic force microscopy (AFM)
dc.subject.ennanoparticle tracking analysis (NTA)
dc.subject.enBrownian motion
dc.titleBrownian Motion Influence on AFM Exosomes’ Size Measurements
dc.typeJournalArticle
dspace.entity.typePublication