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A benzimidazole - based ruthenium (IV) complex inhibits Pseudomonas aeruginosa biofilm formation by interacting with siderophores and the cell envelope, and inducing oxidative stress

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cris.lastimport.scopus2024-02-12T19:48:38Z
dc.abstract.enPseudomonas aeruginosa biofilm-associated infections are a serious medical problem, and new compounds and therapies acting through novel mechanisms are much needed. Herein, the authors report a ruthenium(IV) complex that reduces P. aeruginosa PAO1 biofilm formation by 84%, and alters biofilm morphology and the living-to-dead cell ratio at 1 mM concentration. Including the compound in the culture medium altered the pigments secreted by PAO1, and fluorescence spectra revealed a decrease in pyoverdine. Scanning electron microscopy showed that the ruthenium complex did not penetrate the bacterial cell wall, but accumulated on external cell structures. Fluorescence quenching experiments indicated strong binding of the ruthenium complex to both plasmid DNA and bovine serum albumin. Formamidopyrimidine DNA N-glycosylase (Fpg) protein digestion of plasmid DNA isolated after ruthenium(IV) complex treatment revealed the generation of oxidative stress, which was further proved by the observed upregulation of catalase and superoxide dismutase gene expression.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorCzerwonka, Grzegorz
dc.contributor.authorGmiter, Dawid
dc.contributor.authorGuzy, Anna
dc.contributor.authorRogala, Patrycja
dc.contributor.authorJabłońska-Wawrzycka, Agnieszka
dc.contributor.authorBorkowski, Andrzej
dc.contributor.authorCłapa, Tomasz
dc.contributor.authorNarożna, Dorota
dc.contributor.authorKowalczyk, Paweł
dc.contributor.authorSyczewski, Marcin
dc.contributor.authorDrabik, Marcin
dc.contributor.authorDańczuk, Magdalena
dc.contributor.authorKaca, Wiesław
dc.date.accessioned2024-01-24T17:17:24Z
dc.date.available2024-01-24T17:17:24Z
dc.date.issued2019
dc.description.financeNie dotyczy
dc.description.number1
dc.description.volume3
dc.identifier.doi10.1080/08927014.2018.1564818
dc.identifier.issn0892-7014
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/101428
dc.identifier.weblinkhttp://www.tandfonline.com/doi/abs/10.1080/08927014.2018.1564818?journalCode=gbif20
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofBiofouling
dc.relation.pages59-74
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enRuthenium complex
dc.subject.enbiofilm
dc.subject.enbenzimidazole
dc.subject.enPseudomonas aeruginosa PAO1
dc.subject.enfluorescence quenching
dc.subject.encell surface hydrophobicity
dc.titleA benzimidazole - based ruthenium (IV) complex inhibits Pseudomonas aeruginosa biofilm formation by interacting with siderophores and the cell envelope, and inducing oxidative stress
dc.typeJournalArticle
dspace.entity.typePublication