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Allium Cepa L. response to sodium selenite (Se(IV)) studied in plant roots by a LC-MS-based proteomic approach

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dc.abstract.enLiquid chromatography-high-resolution mass spectrometry was used for the first time to investigate the impact of Se(IV) (10 mgSe L–1 as sodium selenite) on Allium cepa L. root proteome. Using MaxQuant platform, more than 600 proteins were found; 42 were identified based on at least 2 razor + unique peptides, score > 25, and were found to be differentially expressed in the exposed versus control roots with t-test difference > ±0.70 (p < 0.05, Perseus). Se(IV) caused growth inhibition and the decrease of total RNA in roots. Different abundances of proteins involved in transcriptional regulation, protein folding/assembly, cell cycle, energy/carbohydrate metabolism, stress response, and antioxidant defense were found in the exposed vs nonexposed roots. New evidence was obtained on the alteration of sulfur metabolism due to S–Se competition in A. cepa L. which, together with the original analytical approach, is the main scientific contribution of this study. Specifically, proteins participating in assimilation and transformation of both elements were affected; formation of volatile Se compounds seemed to be favored. Changes observed in methionine cycle suggested that Se(IV) stress might repress methylation capability in A. cepa L., potentially limiting accumulation of Se n the form of nonprotein methylated species and affecting adversely transmethylation-dependent signaling pathways.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorWrobel, Katarzyna
dc.contributor.authorEscobosa, Alma Rosa Corrales
dc.contributor.authorWrobel, Kazimierz
dc.contributor.authorBulska, Ewa
dc.contributor.authorKarasiński, Jakub
dc.contributor.authorKonopka, Anna
dc.date.accessioned2024-01-24T15:41:15Z
dc.date.available2024-01-24T15:41:15Z
dc.date.issued2017
dc.description.financeNie dotyczy
dc.description.number19
dc.description.volume65
dc.identifier.doi10.1021/ACS.JAFC.7B01085
dc.identifier.issn0021-8561
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/100462
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofJournal of Agricultural and Food Chemistry
dc.relation.pages3995-4004
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enSelenium
dc.subject.enAllium cepa L.
dc.subject.enabiotic stress
dc.subject.enLC-MS-based proteomics
dc.titleAllium Cepa L. response to sodium selenite (Se(IV)) studied in plant roots by a LC-MS-based proteomic approach
dc.typeJournalArticle
dspace.entity.typePublication